Carbohydrate-active enzymes from the zygomycete fungus Rhizopus oryzae: a highly specialized approach to carbohydrate degradation depicted at genome level

BMC Genomics

Research Article

Pedro M Coutinho¹  Bernard Henrissat¹  Ad Wiebenga²  Joost van den Brink²  Isabelle Benoit³  Evy Battaglia³  Ronald P de Vries⁴ 

[1] Architecture et Fonction des Macromolécules Biologiques, UMR6098, CNRS and Universités d'Aix-Marseille I & II, Case 932, 163 Av de Luminy, 13288, Marseille cedex 9, France;Fungal Physiology, CBS-KNAW, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands;Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584, CH, Utrecht, The Netherlands;Microbiology & Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University, Padualaan 8, 3584, CH, Utrecht, The Netherlands;Fungal Physiology, CBS-KNAW, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands;

关键词: Chitosan;    Chitin;    Pectin;    Glucan;    Inulin;   

DOI  :  10.1186/1471-2164-12-38

 received in 2010-09-22, accepted in 2011-01-17,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundRhizopus oryzae is a zygomycete filamentous fungus, well-known as a saprobe ubiquitous in soil and as a pathogenic/spoilage fungus, causing Rhizopus rot and mucomycoses.ResultsCarbohydrate Active enzyme (CAZy) annotation of the R. oryzae identified, in contrast to other filamentous fungi, a low number of glycoside hydrolases (GHs) and a high number of glycosyl transferases (GTs) and carbohydrate esterases (CEs). A detailed analysis of CAZy families, supported by growth data, demonstrates highly specialized plant and fungal cell wall degrading abilities distinct from ascomycetes and basidiomycetes. The specific genomic and growth features for degradation of easily digestible plant cell wall mono- and polysaccharides (starch, galactomannan, unbranched pectin, hexose sugars), chitin, chitosan, β-1,3-glucan and fungal cell wall fractions suggest specific adaptations of R. oryzae to its environment.ConclusionsCAZy analyses of the genome of the zygomycete fungus R. oryzae and comparison to ascomycetes and basidiomycete species revealed how evolution has shaped its genetic content with respect to carbohydrate degradation, after divergence from the Ascomycota and Basidiomycota.

【 授权许可】

Unknown   
© Battaglia et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.