Identification of prognostic factors in canine mammary malignant tumours: a multivariable survival study

BMC Veterinary Research

Research Article

Célia C Lopes¹  Irina F Amorim¹  Fátima Gärtner²  Joana C Santos³  Jorge R Ribeiro³  Liliana R Martins³  Andreia A Santos⁴  Augusto J Matos⁴ 

[1] Department of Molecular Pathology and Immunology of the Biomedical Sciences Institute of Abel Salazar (ICBAS), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal;Department of Molecular Pathology and Immunology of the Biomedical Sciences Institute of Abel Salazar (ICBAS), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal;Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), R Dr. Roberto Frias, s/n, 4200-465, Porto, Portugal;Department of Veterinary Clinics of the Biomedical Sciences Institute of Abel Salazar (ICBAS), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal;Department of Veterinary Clinics of the Biomedical Sciences Institute of Abel Salazar (ICBAS), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal;Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Largo Professor Abel Salazar, 2, 4099-003, Porto, Portugal;

关键词: Canine;    Mammary;    Tumours, Prognosis;    Multivariable;    Survival;    Study;   

DOI  :  10.1186/1746-6148-9-1

 received in 2011-10-07, accepted in 2013-01-02,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAlthough several histopathological and clinical features of canine mammary gland tumours have been widely studied from a prognostic standpoint, considerable variations in tumour individual biologic behaviour difficult the definition of accurate prognostic factors. It has been suggested that the malignant behaviour of tumours is the end result of several alterations in cellular physiology that culminate in tumour growth and spread. Accordingly, the aim of this study was to determine, using a multivariable model, the independent prognostic value of several immunohistochemically detected tumour-associated molecules, such as MMP-9 and uPA in stromal cells and Ki-67, TIMP-2 and VEGF in cancer cells.ResultsEighty-five female dogs affected by spontaneous malignant mammary neoplasias were followed up for a 2-year post-operative period. In univariate analysis, tumour characteristics such as size, mode of growth, regional lymph node metastases, tumour cell MIB-1 LI and MMP-9 and uPA expressions in tumour-adjacent fibroblasts, were associated with both survival and disease-free intervals. Histological type and grade were related with overall survival while VEGF and TIMP-2 were not significantly associated with none of the outcome parameters. In multivariable analysis, only a MIB-1 labelling index higher than 40% and a stromal expression of MMP-9 higher than 50% retained significant relationships with poor overall and disease-free survival.ConclusionsThe results of this study indicate that MMP-9 and Ki-67 are independent prognostic markers of canine malignant mammary tumours. Furthermore, the high stromal expressions of uPA and MMP-9 in aggressive tumours suggest that these molecules are potential therapeutic targets in the post-operative treatment of canine mammary cancer.

【 授权许可】

Unknown   
© Santos et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.