Fatal infection in three Grey Slender Lorises (Loris lydekkerianus nordicus) caused by clonally related Trueperella pyogenes

BMC Veterinary Research

Case Report

Stefanie P. Glaeser¹  Peter Kämpfer¹  Ellen Prenger-Berninghoff²  Christoph Lämmler³  Samy Nagib³  Osama Sammra³  Amir Abdulmawjood⁴  André Becker⁴  Tobias Eisenberg⁵  Ute Kaim⁵  Christina Geiger⁶  Nicole Schauerte⁶ 

[1] Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, D-35392, Gießen, Germany;Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurter Straße. 85-91, D-35392, Gießen, Germany;Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, D-35392, Gießen, Germany;Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173, Hannover, Germany;Landesbetrieb Hessisches Landeslabor, Schubertstraße. 60, D-35392, Gießen, Germany;Zoologischer Garten Frankfurt am Main, Bernhard-Grzimek-Allee 1, D-60316, Frankfurt, Germany;

关键词: Trueperella pyogenes;    grey slender loris;    Loris lydekkerianus nordicus;    lorises;    virulence genes;    clonal relationship;    DNA fingerprint;    multilocus sequence analysis;   

DOI  :  10.1186/s12917-017-1171-8

 received in 2016-12-15, accepted in 2017-08-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTrueperella pyogenes is a worldwide known bacterium causing mastitis, abortion and various other pyogenic infections in domestic animals like ruminants and pigs. In this study we represent the first case report of three unusual fatal infections of Grey Slender Lorises caused by Trueperella pyogenes. Meanwhile, this study represents the first in-depth description of the multilocus sequence analysis (MLSA) on T. pyogenes species.Case presentationThree Trueperella pyogenes were isolated from three different Grey Slender Lorises, which died within a period of two years at Frankfurt Zoo (Frankfurt am Main - Germany). The three Grey Slender Loris cases were suffering from severe sepsis and died from its complication. During the bacteriological investigation of the three cases, the T. pyogenes were isolated from different organisms in each case. The epidemiological relationship between the three isolates could be shown by four genomic DNA fingerprint methods (ERIC-PCR, BOX-PCR, (GTG)5-PCR, and RAPD-PCR) and by multilocus sequence analysis (MLSA) investigating four different housekeeping genes (fusA-tuf-metG-gyrA).ConclusionIn this study, we clearly showed by means of using three different rep-PCRs, by RAPD-PCR and by MLSA that the genomic fingerprinting of the investigated three T. pyogenes have the same clonal origin and are genetically identical. These results suggest that the same isolate contaminated the animal’s facility and subsequently caused cross infection between the three different Grey Slender Lorises. To the best of our knowledge, this is the first epidemiological approach concentrating on T. pyogenes using MLSA.

【 授权许可】

CC BY   
© The Author(s). 2017