Macrolide-resistant Mycoplasma pneumoniae in adolescents with community-acquired pneumonia

BMC Infectious Diseases

Research Article

Takeyuki Kurihara¹  Niro Okimoto¹  Toshikiyo Hayashi¹  Naoyuki Miyashita¹  Kazunobu Ouchi²  Yasuhiro Kawai²  Hiroto Akaike² 

[1] Department of Internal Medicine I, Kawasaki Medical School, 2-1-80 Nakasange, Kita-ku, 700-8505, Okayama, Japan;Department of Pediatrics, Kawasaki Medical School, 577 Matsushima, 701-0192, Kurashiki, Japan;

关键词: Mycoplasma pneumoniae;    Macrolide-resistant;    Community-acquired pneumonia;    23S rRNA;   

DOI  :  10.1186/1471-2334-12-126

 received in 2011-12-19, accepted in 2012-05-31,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAlthough the prevalence of macrolide-resistant Mycoplasma pneumoniae isolates in Japanese pediatric patients has increased rapidly, there have been no reports concerning macrolide-resistant M. pneumoniae infection in adolescents aged 16 to 19 years old. The purpose of this study was to clarify the prevalence and clinical characteristics of macrolide-resistant M. pneumoniae in adolescent patients with community-acquired pneumonia.MethodsA total of 99 cases with M. pneumoniae pneumonia confirmed by polymerase chain reaction (PCR) and culture were analyzed. Forty-five cases were pediatric patients less than 16 years old, 26 cases were 16 to 19-year-old adolescent patients and 28 cases were adult patients. Primers for domain V of 23S rRNA were used and DNA sequences of the PCR products were compared with the sequence of an M. pneumoniae reference strain.ResultsThirty of 45 pediatric patients (66%), 12 of 26 adolescent patients (46%) and seven of 28 adult patients (25%) with M. pneumoniae pneumonia were found to be infected with macrolide-resistant M. pneumoniae (MR patients). Although the prevalence of resistant strains was similar in pediatric patients between 2008 and 2011, an increase in the prevalence of resistant strains was observed in adolescent patients. Among 30 pediatric MR patients, 26 had an A-to-G transition at position 2063 (A2063G) and four had an A-to-G transition at position 2064 (A2064G). In 12 adolescent MR patients, 10 showed an A2063G transition and two showed an A2064G transition, and in seven adult MR patients, six showed an A2063G transition and one showed an A2064G transition.ConclusionsThe prevalence of macrolide-resistant M. pneumoniae is high among adolescent patients as well as pediatric patients less than 16-years old. To prevent outbreaks of M. pneumoniae infection, especially macrolide-resistant M. pneumoniae, in closed populations including among families, in schools and in university students, physicians should pay close attention to macrolide-resistant M. pneumoniae.

【 授权许可】

CC BY   
© Miyashita et al.; licensee BioMed Central Ltd. 2012