Complete genome of Staphylococcus aureus Tager 104 provides evidence of its relation to modern systemic hospital-acquired strains

BMC Genomics

Research Article

Matthias Nahrendorf¹  Mark R. Liles²  Mohammad J. Hossain²  Andrew D. Brannen³  Richard W. Davis³  Peter Panizzi³  Paul E. Bock⁴  David Mead⁵  Michael Lodes⁵  Scott Monsma⁵ 

[1] Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge St., 02114, Boston, MA, USA;Department of Biological Sciences, Auburn University, 101 Rouse Life Science Building, 36849, Auburn, AL, USA;Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, 4306 Walker Building, 36849, Auburn, AL, USA;Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 37232, Nashville, TN, USA;Lucigen Corporation, 2905 Parmenter St, 53562, Middleton, WI, USA;

关键词: Basic Local Alignment Search Tool;    Minimum Inhibitory Concentration;    Mobile Genetic Element;    Genomic Island;    Illumina MiSeq;   

DOI  :  10.1186/s12864-016-2433-8

 received in 2015-09-28, accepted in 2016-02-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of these MGE, as well as single nucleotide polymorphisms, is of high clinical and microbiological importance. To understand the evolution of these dangerous pathogens, it is paramount to define reference strains that may predate MGE acquisition. One such candidate is S. aureus Tager 104, a previously uncharacterized strain isolated from a patient with impetigo in 1947.ResultsWe show here that S. aureus Tager 104 can survive in the bloodstream and infect naïve organs. We also demonstrate a procedure to construct and validate the assembly of S. aureus genomes, using Tager 104 as a proof-of-concept. In so doing, we bridged confounding gap regions that limited our initial attempts to close this 2.82 Mb genome, through integration of data from Illumina Nextera paired-end, PacBio RS, and Lucigen NxSeq mate-pair libraries. Furthermore, we provide independent confirmation of our segmental arrangement of the Tager 104 genome by the sole use of Lucigen NxSeq libraries filled by paired-end MiSeq reads and alignment with SPAdes software. Genomic analysis of Tager 104 revealed limited MGE, and a νSaβ island configuration that is reminiscent of other hospital acquired S. aureus genomes.ConclusionsTager 104 represents an early-branching ancestor of certain hospital-acquired strains. Combined with its earlier isolation date and limited content of MGE, Tager 104 can serve as a viable reference for future comparative genome studies.

【 授权许可】

CC BY   
© Davis et al. 2016