Microbiota of wild-caught Red Snapper Lutjanus campechanus

BMC Microbiology

Research Article

Covadonga R. Arias¹  William F. Patterson²  Andrea M. Tarnecki³ 

[1] Auburn University, School of Fisheries, Aquaculture, and Aquatic Sciences, 203 Swingle Hall, 36849, Auburn, AL, USA;Department of Marine Sciences, University of South Alabama, Life Sciences Building Room 25, 36688, Mobile, AL, USA;Mote Marine Laboratory, 1600 Ken Thompson Parkway, 34236, Sarasota, FL, USA;

关键词: Microbiota;    Lutjanus campechanus;    Aquaculture;    Pyrosequencing;   

DOI  :  10.1186/s12866-016-0864-7

 received in 2015-12-12, accepted in 2016-10-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe microbiota plays an essential role in host health, particularly through competition with opportunistic pathogens. Changes in total bacterial load and microbiota structure can indicate early stages of disease, and information on the composition of bacterial communities is essential to understanding fish health. Although Red Snapper (Lutjanus campechanus) is an economically important species in recreational fisheries and a primary aquaculture candidate, no information is available on the microbial communities of this species. The aim of this study was to survey the microbiota of apparently healthy, wild-caught Red Snapper from the Gulf of Mexico. Sampled Red Snapper showed no physical signs of disease. Tissues that are either primary entry routes for pathogens (feces, gill) or essential to disease diagnosis (blood) were sampled. Bacteria were enumerated using culture-based techniques and characterized by pyrosequencing.ResultsAerobic counts of feces and gill samples were 107 and 104 CFU g-1, respectively. All individuals had positive blood cultures with counts up to 23 CFU g-1. Gammaproteobacteria dominated the microbiota of all sample types, including the genera Pseudoalteromonas and Photobacterium in feces and Pseudomonas in blood and gill. Gill samples were also dominated by Vibrio while blood samples had high abundances of Nevskia. High variability in microbiota composition was observed between individuals, with percent differences in community composition ranging from 6 to 76 % in feces, 10 to 58 % in gill, and 52 to 64 % in blood.ConclusionsThis study provides the first characterization of the microbiota of the economically significant Red Snapper via pyrosequencing. Its role in fish health highlights the importance of understanding microbiota composition for future work on disease prevention using microbial manipulation.

【 授权许可】

CC BY   
© The Author(s). 2016