Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae(Coleoptera: Curculionidae: Scolytinae)

BMC Genomics

Research Article

Ylva Hillbur¹  Fredrik Schlyter¹  Martin N Andersson²  Jonas M Bengtsson³  Bill S Hansson⁴  Ewald Grosse-Wilde⁴  Christopher I Keeling⁵  Macaire MS Yuen⁵  Maria Li⁵  Jörg Bohlmann⁵ 

[1] Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden;Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden;Department of Biology, Lund University, SE-223 62, Lund, Sweden;Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden;Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy;Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, DE-07745, Jena, Germany;Michael Smith Laboratories, University of British Columbia, V6T 1Z4, Vancouver, BC, Canada;

关键词: Ips typographus;    Dendroctonus ponderosae;    Gene ontology;    Transcriptome;    Odorant receptor;    Ionotropic receptor;    Gustatory receptor;    Odorant binding protein;    Chemosensory Protein;    Sensory neuron membrane protein;   

DOI  :  10.1186/1471-2164-14-198

 received in 2012-10-12, accepted in 2013-03-13,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe European spruce bark beetle, Ips typographus, and the North American mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae), are severe pests of coniferous forests. Both bark beetle species utilize aggregation pheromones to coordinate mass-attacks on host trees, while odorants from host and non-host trees modulate the pheromone response. Thus, the bark beetle olfactory sense is of utmost importance for fitness. However, information on the genes underlying olfactory detection has been lacking in bark beetles and is limited in Coleoptera. We assembled antennal transcriptomes from next-generation sequencing of I. typographus and D. ponderosae to identify members of the major chemosensory multi-gene families.ResultsGene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. Transcripts with terms related to olfactory function were found in both species. Focusing on the chemosensory gene families, we identified 15 putative odorant binding proteins (OBP), 6 chemosensory proteins (CSP), 3 sensory neuron membrane proteins (SNMP), 43 odorant receptors (OR), 6 gustatory receptors (GR), and 7 ionotropic receptors (IR) in I. typographus; and 31 putative OBPs, 11 CSPs, 3 SNMPs, 49 ORs, 2 GRs, and 15 IRs in D. ponderosae. Predicted protein sequences were compared with counterparts in the flour beetle, Tribolium castaneum, the cerambycid beetle, Megacyllene caryae, and the fruit fly, Drosophila melanogaster. The most notable result was found among the ORs, for which large bark beetle-specific expansions were found. However, some clades contained receptors from all four beetle species, indicating a degree of conservation among some coleopteran OR lineages. Putative GRs for carbon dioxide and orthologues for the conserved antennal IRs were included in the identified receptor sets.ConclusionsThe protein families important for chemoreception have now been identified in three coleopteran species (four species for the ORs). Thus, this study allows for improved evolutionary analyses of coleopteran olfaction. Identification of these proteins in two of the most destructive forest pests, sharing many semiochemicals, is especially important as they might represent novel targets for population control.

【 授权许可】

CC BY   
© Andersson et al.; licensee BioMed Central Ltd. 2013