Detection of RUNX2 gene expression in cumulus cells in women undergoing controlled ovarian stimulation

Reproductive Biology and Endocrinology

Research

Spyros Marinopoulos¹  Myrto Papamentzelopoulou¹  Despina Mavrogianni¹  Kostas Kallianidis¹  Dimitris Loutradis¹  Vasiliki Dinopoulou¹  Haralampos Theofanakis¹  Ritsa Bletsa¹  Elli Anagnostou¹  Fotodotis Malamas¹ 

[1] Division of Human Reproduction, IVF Unit, 1st Department of Obstetrics and Gynaecology, Alexandra Hospital, Athens University Medical School, Athens, Greece;

关键词: RUNX2 expression;    ART treatment;    Cumulus cells;    Estradiol levels;    ICSI;   

DOI  :  10.1186/1477-7827-10-99

 received in 2012-06-28, accepted in 2012-11-24,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundRUNX2 is a transcription factor, whose expression has been recently identified in the mouse ovary. Regulation of RUNX2 expression and its function in the human ovary have not been determined yet. The aim of the present study is the investigation of the possible correlation between RUNX2 gene expression in cumulus cells and controlled ovarian stimulation and pregnancy outcomes after ART treatment.MethodsA total of 41 patients undergoing ICSI treatment for male factor infertility were enrolled into a specific ART program, during which cumulus cells were collected. The expression of RUNX2 gene in cumulus cells was examined by real-time PCR.ResultsConcerning RUNX2 gene expression, 12 out of 41 women were detected with RUNX2 expression, with ratios ranging from 0.84 to 1.00, while 28 out of 41 women had no expression (ratio = 0). Only 1 woman presented a weak RUNX2 gene expression (ratio = 0.52). From 8 women that proceeded to pregnancy, 7 of them did not express RUNX2 gene in cumulus cells, while one was the woman with weak gene expression that also achieved pregnancy. The group of women without RUNX2 expression presented higher number of follicles (p = 0.013), higher number of retrieved oocytes (p = 0.016), higher basal LH serum levels (p = 0.016) and higher peak estradiol levels (p = 0.013), while the number of fertilized oocytes differed marginally between the two groups (p = 0.089). Moreover, RUNX2 expression was negatively associated with LH levels (OR = 0.22, p = 0.021) and E2 levels (OR = 0.25, p = 0.026).ConclusionsConsequently, based on the preliminary findings of the present pilot study a potential inhibitory mechanism of RUNX2 gene is observed in the ovary when high mRNA levels are detected, suggesting that RUNX2 could possibly be used as a candidate genetic marker in the monitoring of the outcome of an ART treatment.

【 授权许可】

Unknown   
© Papamentzelopoulou et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.