Thymosin β4 coated nanofiber scaffolds for the repair of damaged cardiac tissue

Journal of Nanobiotechnology

Research

Mehir Desai¹  Arjun Patel²  Louise Duvalsaint³  Arun Kumar⁴  Edward D Marks⁴ 

[1] Department of Biochemistry, University of Delaware, 19716, Newark, DE, USA;Department of Biological Sciences, University of Delaware, 19716, Newark, DE, USA;Department of Biomedical Engineering, University of Delaware, 19716, Newark, DE, USA;Nanomedicine Research Laboratory, Department of Medical Laboratory Science, College of Health Sciences, University of Delaware, 19716, Newark, DE, USA;

关键词: Biocompatibility;    FTIR;    Heart;    Polycaprolactone;    Scaffold;   

DOI  :  10.1186/1477-3155-12-10

 received in 2014-01-22, accepted in 2014-03-04,  发布年份 2014

来源: Springer

【 摘 要 】

After a cardiac event, proper treatment and care of the damaged tissue is crucial in restoring optimal cardiac function and preventing future cardiac events. Recently, thymosin β4 has been found to play a vital role in cardiac cell health and development by regulating angiogenesis, inflammatory responses, and wound healing. We proposed that defined poly(ϵ-caprolactone) (PCL) nanoscaffolds coated with thymosin β4 could efficiently differentiate murine-derived cardiomyocytes into functioning cardiac tissue. PCL nanoscaffolds were developed through electrospinning technology, and subsequently coated with a thymosin β4 solution. Cardiomyocytes were seeded on coated and uncoated nanoscaffolds and observed for six days via fluorescent and electron microscopy. Our results demonstrated a robust growth and differentiation of cardiomyocytes on coated nanoscaffolds compared with uncoated, showing potential for nanoscaffold-mediated cardiac cell replacement in vivo after an MI or other cardiac event.

【 授权许可】

CC BY   
© Kumar et al.; licensee BioMed Central Ltd. 2014