Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

BMC Infectious Diseases

Research Article

Takashi Matsuba¹  Yasuhiko Suzuki²  Chie Nakajima²  Toshio Hattori³  Jingge Zhao⁴  Haorile Chagan-Yasutan⁴  Elizabeth Freda Telan⁵  Susan Leano⁵  Xiaoyan Zhang⁶ 

[1] Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, 683-8503, Tottori, Japan;Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, 001-0020, Sapporo, Hokkaido, Japan;The Global Station for Zoonosis Control, Hokkaido University Global Institution for Collaborative Research and Education, 001-0020, Sapporo, Hokkaido, Japan;Graduate School of Health Science Studies, Kibi International University, 8 Igamachi, 716-8508, Takahashi, Japan;Laboratory of Disaster Medicine, International Research Institute of Disaster Science, Tohoku University, 980-8574, Sendai, Miyagi, Japan;STD AIDS Cooperative Central Laboratory, San Lazaro Hospital, Quiricada Street, 1003, Manila, Philippines;Shanghai Public Health Clinical Center, Fudan Univeristy, 201508, Shanghai, China;

关键词: Mycobacterium tuberculosis;    Dimorphic antigen;    Rv0679c;    Beijing genotype;    Philippines;    Immunoglobulin G;    Immunoglobulin A;   

DOI  :  10.1186/s12879-017-2442-5

 received in 2017-01-04, accepted in 2017-05-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundStrains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses. However, the immune response to Rv0679c in humans remains unknown. Therefore, we aimed to investigate the anti-Rv0679c immune response in TB patients from the endemic and non-endemic regions of the Beijing MTB genotype.MethodsWe analyzed the Rv0679c-specific antibody responses in 84 subjects from the endemic region of the Beijing genotype MTB in China, including 45 pulmonary TB patients (C-PTB) and 39 healthy controls (C-HC), and 81 subjects from the Philippines (the endemic region of the non-Beijing genotype), including 51 pulmonary TB patients (P-PTB) and 30 healthy controls (P-HC). Anti-tuberculous-glycolipid (TBGL) antigen was used as the control antibody.ResultsTBGL IgG titers were higher in both C-PTB and P-PTB than those in their corresponding HC (C-PTB median 4.2, P-PTB median 11.2; C-PTB vs. P-PTB, p > 0.05), suggesting immune response comparability in PTB from two different countries. C-PTB showed a higher response compared to C-HC for anti-K-type IgG (53.3%) than anti-N-type IgG (6.67%); this response was not observed in P-PTB (both N-type and K-type 9.80%).ConclusionDimorphic antigen Rv0679c was found to be associated with distinct immune response patterns, indicating the role of Beijing/non-Beijing genotype of MTB in stimulating specific responses in TB patients from the endemic region of Beijing MTB. Meanwhile, reactions to Rv0679c in patients and HC from non-endemic regions of the Beijing MTB may be caused by the response to the common epitope of Rv0679c N/K-type.

【 授权许可】

CC BY   
© The Author(s). 2017