期刊论文详细信息
BMC Cell Biology
Dynamic expression of synemin isoforms in mouse embryonic stem cells and neural derivatives
Research Article
Zhenlin Li1  Denise Paulin1  Zhigang Xue1  Nicolas Diguet1  Sheila C de Souza Martins2  Jean-Christophe Larcher3  Onnik Agbulut4  Stevens K Rehen5  Bruna S Paulsen5  Vivaldo Moura-Neto5 
[1] Department of Aging, Stress and Inflammation, UPMC Sorbonne University, Paris, France;Department of Aging, Stress and Inflammation, UPMC Sorbonne University, Paris, France;Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil;Department of Developmental Biology, UPMC Sorbonne University, UMR 7622 CNRS, Paris, France;Department of Functional and Adaptive Biology, Paris Diderot University, Paris, France;Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil;
关键词: Embryonic Stem Cell;    Glial Fibrillary Acidic Protein;    Neural Stem Cell;    Neural Differentiation;    Mouse Embryonic Stem Cell;   
DOI  :  10.1186/1471-2121-12-51
 received in 2011-04-07, accepted in 2011-11-23,  发布年份 2011
来源: Springer
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【 摘 要 】

BackgroundIntermediate filaments (IFs) are major components of the mammalian cytoskeleton and expressed in cell-type-specific patterns. Morphological changes during cell differentiation are linked to IF network remodeling. However, little is known concerning the presence and the role of IFs in embryonic stem (ES) cells and during their differentiation.ResultsWe have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. Synemin H was produced only in neural precursors when neural differentiation started, concurrently with synemin M, nestin and glial fibrillary acidic protein. However, both synemin H and M were restricted to the progenitor line during the neural differentiation program. Our in vivo analysis also confirmed the expression of synemins H/M in multipotent neural stem cells in the subventricular zone of the adult brain, a neurogenic germinal niche of the mice. Knocking down synemin in ES cells by shRNA lentiviral particles transduction has no influence on expression of Oct4, Nanog and SOX2, but decreased keratin 8 expression.ConclusionsOur study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells.

【 授权许可】

Unknown   
© de Souza Martins et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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