Hyper secretion of Thermobifida fusca β-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans

Microbial Cell Factories

Research

Tsutomu Tanaka¹  Shuhei Noda¹  Takaya Miyazaki¹  Akihiko Kondo² 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, 657-8501, Nada, Kobe, Japan;Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, 657-8501, Nada, Kobe, Japan;Biomass Engineering Program, RIKEN, 1-7-22 Suehiro-cho, Kanagawa, 230-0045, Tsurumi-ku, Yokohama, Japan;Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, 136-713, Seoul, Republic of Korea;

关键词: Streptomyces;    Protein secretion;    Signal peptide sequence;    Tat pathway;   

DOI  :  10.1186/1475-2859-12-88

 received in 2013-07-02, accepted in 2013-09-28,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundProtein production as secretory-form is a powerful tool in industrial enzyme production due to the simple purification procedure. Streptomyces lividans is a versatile host for secretory production of useful proteins. In order to expand the amount of secreted protein, signal peptide sequences, which encourage protein secretion from inside cell to extracellular environment, are one of the most significant factors. In this study, we focused on Streptomyces lividans as a host strain to secrete useful proteins, and screened for signal peptides from the biomass-degradation enzymes derived from Thermobifida fusca YX and S. lividans.ResultsThree candidate signal peptides were isolated and evaluated for their protein secretion ability using β-glucosidase derived from T. fusca YX, which is a non-secreted protein, as a model protein. Using S. lividans xylanase C signal peptide, the amount of produced the β-glucosidase reached 10 times as much as that when using Streptomyces cinnamoneus phospholipase D signal peptide, which was identified as a versatile signal peptide in our previous report. In addition, the introduction of the β-glucosidase fused to xylanase C signal peptide using two kinds of plasmid, pUC702 and pTYM18, led to further protein secretion, and the maximal level of produced the β-glucosidase increased up to 17 times (1.1 g/l) compared to using only pUC702 carrying the β-glucosidase fused to S. cinnamoneus phospholipase D signal peptide.ConclusionIn the present study, we focused on signal peptide sequences derived from biomass degradation enzymes, which are usually secreted into the culture supernatant, and screened for signal peptides leading to effective protein secretion. Using the signal peptides, the hyper-protein secretion system was successfully demonstrated for the cytoplasmic β-glucosidase.

【 授权许可】

Unknown   
© Miyazaki et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.