Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern Vietnam

BMC Microbiology

Research Article

Jaap A. Wagenaar¹  Constance Schultsz²  Nguyen Vinh Trung²  Pham Van Minh³  Nguyen Thi Nhung³  Hoang Ngoc Nhung³  Ha Thanh Tuyen³  Ngo Thi Hoa⁴  James Campbell⁴  Juan J. Carrique-Mas⁴  Nguyen Thi Nhu Mai⁵  Ho Huynh Mai⁶  Thai Quoc Hieu⁶ 

[1]Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[2]Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands

[3]Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

[4]Department of Global Health-Amsterdam Institute for Global Health and Development, Amsterdam, The Netherlands

[5]Oxford University Clinical Research Unit, Centre for Tropical Medicine, Ho Chi Minh City, Vietnam

[6]Oxford University Clinical Research Unit, Centre for Tropical Medicine, Ho Chi Minh City, Vietnam

[7]Oxford University Clinical Research Unit, Centre for Tropical Medicine, Ho Chi Minh City, Vietnam

[8]Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK

[9]Preventive Medicine Center, My Tho, Tien Giang, Vietnam

[10]Sub-Department of Animal Health, My Tho, Tien Giang, Vietnam

关键词: EAEC;    STEC;    E. coli;    Chicken;    Humans;    Vietnam;   

DOI  :  10.1186/s12866-016-0827-z

 received in 2016-02-20, accepted in 2016-08-31,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundEnteroaggregative (EAEC) and Shiga-toxin producing Escherichia coli (STEC) are a major cause of diarrhea worldwide. E. coli carrying both virulence factors characteristic for EAEC and STEC and producing extended-spectrum beta-lactamase caused severe and protracted disease during an outbreak of E. coli O104:H4 in Europe in 2011. We assessed the opportunities for E. coli carrying the aggR and stx genes to emerge in ‘backyard’ farms in south-east Asia.ResultsFaecal samples collected from 204 chicken farms; 204 farmers and 306 age- and gender-matched individuals not exposed to poultry farming were plated on MacConkey agar plates with and without antimicrobials being supplemented. Sweep samples obtained from MacConkey agar plates without supplemented antimicrobials were screened by multiplex PCR for the detection of the stx1, stx2 and aggR genes. One chicken farm sample each (0.5 %) contained the stx1 and the aggR gene. Eleven (2.4 %) human faecal samples contained the stx1 gene, 2 samples (0.4 %) contained stx2 gene, and 31 (6.8 %) contained the aggR gene. From 46 PCR-positive samples, 205 E. coli isolates were tested for the presence of stx1, stx2, aggR, wzxO104 and fliCH4 genes. None of the isolates simultaneously contained the four genetic markers associated with E. coli O104:H4 epidemic strain (aggR, stx2, wzxO104 and fliCH4). Of 34 EAEC, 64.7 % were resistant to 3rd-generation cephalosporins.ConclusionThese results indicate that in southern Vietnam, the human population is a more likely reservoir of aggR and stx gene carrying E. coli than the chicken population. However, conditions for transmission of isolates and/or genes between human and animal reservoirs resulting in the emergence of highly virulent E. coli strains are still favorable, given the nature of‘backyard’ farms in Vietnam.

【 授权许可】

CC BY   
© The Author(s). 2016