Effect of α-linolenic acid on endoplasmic reticulum stress-mediated apoptosis of palmitic acid lipotoxicity in primary rat hepatocytes

Lipids in Health and Disease

Research

Jian Bai¹  Lei Dong²  Yong Zhang³  Hongyang Shi⁴  Xia Yang⁴ 

[1] Department of Clinical Medicine, Xi'an Medical University, No. 1, Xin Wang Road, 710021, Xi'an, Shaanxi Province, China;Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China;Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China;Anesthesia Department, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China;Department of Respiration, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, West 5th Road, 710004, Xi'an, Shaanxi Province, China;

关键词: Palmitic Acid;    Endoplasmic Reticulum Stress;    Unfold Protein Response;    Nonalcoholic Fatty Liver Disease;    Tunicamycin;   

DOI  :  10.1186/1476-511X-10-122

 received in 2011-06-08, accepted in 2011-07-25,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundHepatic inflammation and degeneration induced by lipid depositions may be the major cause of nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the effects of saturated and unsaturated fatty acids (FA) on apoptosis in primary rat hepatocytes.MethodsThe primary rat hepatocytes were treated with palmitic acid and/or α-linolenic acid in vitro. The expression of proteins associated with endoplasmic reticulum (ER) stress, apoptosis, caspase-3 levels were detected after the treatment.ResultsThe treatment with palmitic acid produced a significant increase in cell death. The unfolded protein response (UPR)-associated genes CHOP, GRP78, and GRP94 were induced to higher expression levels by palmitic acid. Co-treatment with α-linolenic acid reversed the apoptotic effect and levels of all three indicators of ER stress exerted by palmitic acid. Tunicamycin, which induces ER stress produced similar effects to those obtained using palmitic acid; its effects were also reversed by α-linolenic acid.Conclusionsα-Linolenic acid may provide a useful strategy to avoid the lipotoxicity of dietary palmitic acid and nutrient overload accompanied with obesity and NAFLD.

【 授权许可】

Unknown   
© Zhang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.