The beneficial effects of exercise in rodents are preserved after detraining: a phenomenon unrelated to GLUT4 expression

Cardiovascular Diabetology

Original Investigation

Natalia M Leguisamo¹  Melissa M Markoski¹  Alexandre M Lehnen¹  Beatriz Schaan²  Graziela H Pinto³  Ubiratan F Machado⁴  Kátia De Angelis⁵ 

[1] Instituto de Cardiologia do Rio Grande do Sul/Fundação Universitária de Cardiologia, Rio Grande do Sul, Brasil;Instituto de Cardiologia do Rio Grande do Sul/Fundação Universitária de Cardiologia, Rio Grande do Sul, Brasil;Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do, Rio Grande do Sul, Brasil;Instituto de Cardiologia do Rio Grande do Sul/Fundação Universitária de Cardiologia, Rio Grande do Sul, Brasil;Universidade Federal de Ciências da Saúde de Porto Alegre, Rio Grande do Sul, Brasil;Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil;Laboratório do Movimento Humano, Universidade São Judas Tadeu, São Paulo, Brasil;

关键词: Exercise Training;    Gastrocnemius Muscle;    Insulin Tolerance Test;    GLUT4 Expression;    Maximal Exercise Test;   

DOI  :  10.1186/1475-2840-9-67

 received in 2010-08-20, accepted in 2010-10-28,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAlthough exercise training has well-known cardiorespiratory and metabolic benefits, low compliance with exercise training programs is a fact, and the harmful effects of physical detraining regarding these adaptations usually go unnoticed. We investigated the effects of exercise detraining on blood pressure, insulin sensitivity, and GLUT4 expression in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY).MethodsStudied animals were randomized into sedentary, trained (treadmill running/5 days a week, 60 min/day for 10 weeks), 1 week of detraining, and 2 weeks of detraining. Blood pressure (tail-cuff system), insulin sensitivity (kITT), and GLUT4 (Western blot) in heart, gastrocnemius and white fat tissue were measured.ResultsExercise training reduced blood pressure (19%), improved insulin sensitivity (24%), and increased GLUT4 in the heart (+34%); gastrocnemius (+36%) and fat (+22%) in SHR. In WKY no change in either blood pressure or insulin sensitivity were observed, but there was an increase in GLUT4 in the heart (+25%), gastrocnemius (+45%) and fat (+36%) induced by training. Both periods of detraining did not induce any change in neither blood pressure nor insulin sensitivity in SHR and WKY. One-week detraining reduced GLUT4 in SHR (heart: -28%; fat: -23%) and WKY (heart: -19%; fat: -22%); GLUT4 in the gastrocnemius was reduced after a 2-week detraining (SHR: -35%; WKY: -25%). There was a positive correlation between GLUT4 (gastrocnemius) and the maximal velocity in the exercise test (r = 0.60, p = 0.004).ConclusionsThe study findings show that in detraining, despite reversion of the enhanced GLUT4 expression, cardiorespiratory and metabolic beneficial effects of exercise are preserved.

【 授权许可】

CC BY   
© Lehnen et al; licensee BioMed Central Ltd. 2010