Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China

BMC Infectious Diseases

Research Article

Yuesheng Li¹  Weiwei Xing²  Kui Sun²  Wenliang Fu²  Donggang Xu²  Yuanyuan Wang²  Minji Zou²  Wenrong Xia²  Hongbin He³  Xinling Yu³  Jingtao Feng³  Zhihong Luo³ 

[1] Queensland Institute of Medical Research, Brisbane, Australia;The Laboratory of genomic engineering, Beijing Institute of Basic Medical Sciences, Beijing, China;The key laboratory of Immune and Control of Schistosomiasis, Hunan Institute of Parasitic Diseases, Hunan, China;

关键词: Schistosoma japonicum;    Recombinase polymerase amplification;    Field evaluation;    Diagnosis;   

DOI  :  10.1186/s12879-017-2182-6

 received in 2016-06-17, accepted in 2017-01-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCurrent diagnostic methods for Schistosoma japonicum infection are insensitive for low-density infections. Therefore, a new diagnostic assay based on recombinase polymerase amplification (RPA) technology was established and assessed for field applification.MethodsThe S.japonicum RPA assay was developed to target highly repetitive retrotransposon SjR2 gene of S japonicum, and its sensitivity and specificity were assessed by serial dilution of S. japonicum genomic DNA and other related worm genomic DNA respectively. The RPA diagnostic validity was first evaluated in 60 fecal samples from healthy people and patients, and then compared with other diagnostic tests in 200 high-risk individuals living in endemic areas.ResultsThe real time RPA assay could detect 0.9 fg S. japonicum DNA within 15 min and distinguish S. japonicum from other worms. The validity analysis of RPA for the detection of S. japonicum in stool samples from 30 S. japonicum-infected patients and 30 healthy persons indicated 100% sensitivity and specificity. When testing 200 fecal or serum samples from a high-risk population, the percentage sensitivity of RPA was 100%, whereas that of indirect hemagglutination assay (IHA) and enzyme-linked immunosorbent assay (ELISA) were 80.3% and 85.2% respectively. In addition, the RPA presented better consistency with the stool-based tests than IHA and ELISA. Overall, the RPA was superior to other detection methods with respect to detection time, sensitivity, and convenience.ConclusionsThis is the first time we applied the RPA technology to the field evaluation of S. japonicum infection. And the results suggest that RPA-based assays can be used as a promising point-of-care test for the diagnosis of schistosomiasis.

【 授权许可】

CC BY   
© The Author(s). 2017