Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system

BMC Medical Imaging

Research Article

Rolf Gebker¹  Kristof Graf¹  Thomas Hucko¹  Thore Dietrich¹  Cosima Jahnke²  Ingo Paetsch²  Robert Manka³  Bernhard Schnackenburg⁴ 

[1] Department of Cardiology, German Heart Institute Berlin, Berlin, Germany;Department of Cardiology, University Hospital RWTH Aachen, Aachen, Germany;Department of Cardiology, University Hospital Zurich, Rämistrasse 100, 8091, Zurich, Switzerland;Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland;Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich, Switzerland;Philips Healthcare, Hamburg, Germany;

关键词: Cardiac Magnetic Resonance;    Myocardial Infarct Size;    Magnetic Resonance System;    Small Animal Imaging;    Cardiac Magnetic Resonance Study;   

DOI  :  10.1186/1471-2342-13-44

 received in 2013-05-31, accepted in 2013-12-04,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundTo evaluate the inter-study, inter-reader and intra-reader reproducibility of cardiac cine and scar imaging in rats using a clinical 3.0 Tesla magnetic resonance (MR) system.MethodsThirty-three adult rats (Sprague–Dawley) were imaged 24 hours after surgical occlusion of the left anterior descending coronary artery using a 3.0 Tesla clinical MR scanner (Philips Healthcare, Best, The Netherlands) equipped with a dedicated 70 mm solenoid receive-only coil. Left-ventricular (LV) volumes, mass, ejection fraction and amount of myocardial scar tissue were measured. Intra-and inter-observer reproducibility was assessed in all animals. In addition, repeat MR exams were performed in 6 randomly chosen rats within 24 hours to assess inter-study reproducibility.ResultsThe MR imaging protocol was successfully completed in 32 (97%) animals. Bland-Altman analysis demonstrated high intra-reader reproducibility (mean bias%: LV end-diastolic volume (LVEDV), -1.7%; LV end-systolic volume (LVESV), -2.2%; LV ejection fraction (LVEF), 1.0%; LV mass, -2.7%; and scar mass, -1.2%) and high inter-reader reproducibility (mean bias%: LVEDV, 3.3%; LVESV, 6.2%; LVEF, -4.8%; LV mass, -1.9%; and scar mass, -1.8%). In addition, a high inter-study reproducibility was found (mean bias%: LVEDV, 0.1%; LVESV, -1.8%; LVEF, 1.0%; LV mass, -4.6%; and scar mass, -6.2%).ConclusionsCardiac MR imaging of rats yielded highly reproducible measurements of cardiac volumes/function and myocardial infarct size on a clinical 3.0 Tesla MR scanner system. Consequently, more widely available high field clinical MR scanners can be employed for small animal imaging of the heart e.g. when aiming at serial assessments during therapeutic intervention studies.

【 授权许可】

CC BY   
© Manka et al.; licensee BioMed Central Ltd. 2013