Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed

BMC Plant Biology

Research Article

Hui-Zhe Chen¹  Wen-Zhen Liu¹  Ya-Ping Fu¹  Shu-Zhen Wang²  Hua-Sheng Ma²  Shi-Heng Wang²  Ya Xin²  Jian-Xin Tong²  Fang Wang²  Song-Lin Ruan³ 

[1] National Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, PR China;Plant Molecular Biology & Proteomics Lab, Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, 310024, Hangzhou, PR China;Plant Molecular Biology & Proteomics Lab, Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, 310024, Hangzhou, PR China;National Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, PR China;

关键词: Salt Stress;    Transgenic Line;    Salt Tolerance;    Protein Spot;    Rice Seedling;   

DOI  :  10.1186/1471-2229-11-34

 received in 2010-06-07, accepted in 2011-02-16,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundHigh Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis of hybrid rice shoot proteins from Shanyou 10 seedlings, a salt-tolerant hybrid variety, and Liangyoupeijiu seedlings, a salt-sensitive hybrid variety, was performed to identify new components involved in salt-stress signaling.ResultsPhenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings. Interestingly, wild-type seedlings exhibited a marked reduction in maximal photochemical efficiency under salt stress, whereas no such change was observed for OsCYP2-transgenic seedlings. OsCYP2-transgenic seedlings had lower levels of lipid peroxidation products and higher activities of antioxidant enzymes than wild-type seedlings. Spatiotemporal expression analysis of OsCYP2 showed that it could be induced by salt stress in both Shanyou 10 and Liangyoupeijiu seedlings, but Shanyou 10 seedlings showed higher OsCYP2 expression levels. Moreover, circadian rhythm expression of OsCYP2 in Shanyou 10 seedlings occurred earlier than in Liangyoupeijiu seedlings. Treatment with PEG, heat, or ABA induced OsCYP2 expression in Shanyou 10 seedlings but inhibited its expression in Liangyoupeijiu seedlings. Cold stress inhibited OsCYP2 expression in Shanyou 10 and Liangyoupeijiu seedlings. In addition, OsCYP2 was strongly expressed in shoots but rarely in roots in two rice hybrid varieties.ConclusionsTogether, these data suggest that OsCYP2 may act as a key regulator that controls ROS level by modulating activities of antioxidant enzymes at translation level. OsCYP2 expression is not only induced by salt stress, but also regulated by circadian rhythm. Moreover, OsCYP2 is also likely to act as a key component that is involved in signal pathways of other types of stresses-PEG, heat, cold, or ABA.

【 授权许可】

CC BY   
© Ruan et al; licensee BioMed Central Ltd. 2011