Immunohistochemical localization of hepatopancreatic phospholipase A2in Hexaplex Trunculus digestive cells

Lipids in Health and Disease

Research

Tarek Rebai¹  Nicholas Boulais²  Laurent Misery²  Youssef Gargouri³  Hafedh Mejdoub³  Aida Karray³  Zied Zarai³  Sofiane Bezzine³ 

[1] Laboratoire d'Histoembryologie, FMS, Université de Sfax, BPW 3029, Tunisia;Laboratoire de Neurobiologie Cutanée, CHU Morvan, Université de Brest, 29609, BREST cedex, France;Laboratoire de biochimie et de génie enzymatique des lipases, Université de Sfax, ENIS BPW 1173, Tunisia;

关键词: Bile Salt;    Digestive Gland;    Food Digestion;    Endocytic Vesicle;    Digestive Cell;   

DOI  :  10.1186/1476-511X-10-91

 received in 2011-02-25, accepted in 2011-06-01,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMammalian sPLA2-IB localization cell are well characterized. In contrast, much less is known about aquatic primitive ones. The aquatic world contains a wide variety of living species and, hence represents a great potential for discovering new lipolytic enzymes and the mode of digestion of lipid food.ResultsThe marine snail digestive phospholipase A2 (mSDPLA2) has been previously purified from snail hepatopancreas. The specific polyclonal antibodies were prepared and used for immunohistochimical and immunofluorescence analysis in order to determine the cellular location of mSDPLA2. Our results showed essentially that mSDPLA2 was detected inside in specific vesicles tentatively named (mSDPLA2+) granules of the digestive cells. No immunolabelling was observed in secretory zymogene-like cells. This immunocytolocalization indicates that lipid digestion in the snail might occur in specific granules inside the digestive cells.ConclusionThe cellular location of mSDPLA2 suggests that intracellular phospholipids digestion, like other food components digestion of snail diet, occurs in these digestive cells. The hepatopancreas of H. trunculus has been pointed out as the main region for digestion, absorption and storage of lipids.

【 授权许可】

Unknown   
© Zarai et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.