Expression of aldo-keto reductase family 1 member C1 (AKR1C1)gene in porcine ovary and uterine endometrium during the estrous cycle and pregnancy

Reproductive Biology and Endocrinology

Research

Seong-Jo Yun¹  Jong-Ju Park¹  Cha-Won Park¹  Jong-Taek Yoon¹  Kwan-Sik Min¹  Purevjargal Naidansuren¹  Tseeleema Nanjidsuren¹  Sue-Yun Hwang¹  Sang-Hwan Kim¹  Bo-Woong Sim¹  Kyeong-Seok Seo¹  Nam-Hyung Kim²  Myung-Hwa Kang³  Keitaro Yamanouchi⁴  Heewon Seo⁵  Hakhyun Ka⁵ 

[1] Animal Biotechnology, Graduate School of Bio & Information Technology, Institute of Genetic Engineering, Hankyong National University,, 456-749,, Ansung, Korea;Department of Animal Science, Chungbuk National University,, 361-763,, Cheongju, Korea;Department of Food and Nutrition, Hoseo University, 336-795,, Asan, Korea;Department of Veterinary Physiology, Veterinary Medical Science, The University of Tokyo,, 113-8657,, Tokyo, Japan;Division of Biological Science and Technology, Yonsei University,, 220-710,, Wonju, Korea;

关键词: Corpus Luteum;    Estrous Cycle;    Ovulation Rate;    Luteal Cell;    Uterine Endometrium;   

DOI  :  10.1186/1477-7827-9-139

 received in 2011-07-24, accepted in 2011-10-20,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe aldo-keto reductase family 1 member C1 (AKR1C1) belongs to a superfamily of NADPH-dependent reductases that convert a wide range of substrates, including carbohydrates, steroid hormones, and endogenous prostaglandins. The 20alpha-hydroxysteroid dehydrogenase (20alpha-HSD) is a member of AKR family. The aims of this study were to determine its expression in the ovary and uterus endometrium during the estrous cycle and pregnancy.MethodsRapid amplification of cDNA ends (RACE) experiments were performed to obtain the 5' and 3' ends of the porcine 20alpha-HSD cDNA. Reverse-transcriptase-PCR (RT-PCR), real-time PCR, northern blot analysis, and western blot analysis were performed to examine the expression of porcine 20alpha-HSD. Immunohistochemical analysis was also performed to determine the localization in the ovary.ResultsThe porcine 20alpha-HSD cDNA is 957 bp in length and encodes a protein of 319 amino acids. The cloned cDNA was virtually the same as the porcine AKR1C1 gene (337 amino acids) reported recently, and only differed in the C-terminal region (the AKR1C1 gene has a longer C-terminal region than our sequence). The 20alpha-HSD gene (from now on referred to as AKR1C1) cloned in this paper encodes a deletion of 4 amino acids, compared with the C-terminal region of AKR1C1 genes from other animals. Porcine AKR1C1 mRNA was expressed on day 5, 10, 12, 15 of the cycle and 0-60 of pregnancy in the ovary. The mRNA was also specifically detected in the uterine endometrium on day 30 of pregnancy. Western blot analysis indicated that the pattern of AKR1C1 protein in the ovary during the estrous cycle and uterus during early pregnancy was similar to that of AKR1C1 mRNA expression. The recombinant protein produced in CHO cells was detected at approximately 37 kDa. Immunohistochemical analysis also revealed that pig AKR1C1 protein was localized in the large luteal cells in the early stages of the estrous cycle and before parturition.ConclusionsOur study demonstrated that AKR1C1 mRNA and protein are coordinately expressed in the luteal cell of ovary throughout the estrous cycle and in the uterus on day 30 of pregnancy. Thus, the porcine AKR1C1 gene might control important mechanisms during the estrous cycle.

【 授权许可】

Unknown   
© Seo et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.