ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum

BMC Microbiology

Research Article

Johannes Schmidpeter¹  Marlis Dahl¹  Jörg Hofmann¹  Christian Koch¹ 

[1] Department of Biology, Division of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstrasse 5, 91058, Erlangen, Germany;

关键词: Phytopathogenic ascomycete fungus;    ATMT;    Colletotrichum higginsianum;    Conidiation;    NDR Kinase Cbk1;    Mob1;    Mob2;    Mob3;    Ace2;    Ssd1;    Cts1;   

DOI  :  10.1186/s12866-017-0932-7

 received in 2016-09-08, accepted in 2017-01-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that control crucial cellular processes like cytokinesis, proliferation and morphology.ResultsWe identified a hypomorphic allele of ChMOB2 in a random insertional mutant (vir-88) of the hemibiotrophic ascomycete fungus Colletotrichum higginsianum. The mutant is impaired in conidiation, host penetration and virulence on Arabidopsis thaliana. ChMob2 binds to and co-localizes with the NDR/LATS kinase homolog ChCbk1. Mutants in the two potential ChCbk1 downstream targets ChSSD1 and ChACE2 show defects in pathogenicity. The genome of C. higginsianum encodes two more Mob proteins. While we could not detect any effect on pathogenicity in ΔChmob3 mutants, ChMob1 is involved in conidiation, septae formation and virulence.ConclusionThis study shows that ChMob2 binds to the conserved NDR/LATS Kinase ChCbk1 and plays an important role in pathogenicity of Colletotrichum higginsianum on Arabidopsis thaliana.

【 授权许可】

CC BY   
© The Author(s). 2017