caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts

BMC Bioinformatics

Research Article

Andrew N Young¹  Qiqin Yin-Goen¹  John H Phan²  James H Torrance²  R Mitchell Parry²  Richard A Moffitt²  Todd H Stokes²  May D Wang³ 

[1] Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Grady Health System, Grady Memorial Hospital, 30303, Atlanta, GA, USA;The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, 30332, Atlanta, GA, USA;The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, 30332, Atlanta, GA, USA;Department of Electrical and Computer Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA;Winship Cancer Institute, Emory University, 30322, Atlanta, GA, USA;

关键词: Singular Value Decomposition;    Outlier Probe;    Outlier Detection;    Probe Intensity;    Quantile Normalization;   

DOI  :  10.1186/1471-2105-12-383

 received in 2011-02-01, accepted in 2011-09-29,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundIn previous work, we reported the development of caCORRECT, a novel microarray quality control system built to identify and correct spatial artifacts commonly found on Affymetrix arrays. We have made recent improvements to caCORRECT, including the development of a model-based data-replacement strategy and integration with typical microarray workflows via caCORRECT's web portal and caBIG grid services. In this report, we demonstrate that caCORRECT improves the reproducibility and reliability of experimental results across several common Affymetrix microarray platforms. caCORRECT represents an advance over state-of-art quality control methods such as Harshlighting, and acts to improve gene expression calculation techniques such as PLIER, RMA and MAS5.0, because it incorporates spatial information into outlier detection as well as outlier information into probe normalization. The ability of caCORRECT to recover accurate gene expressions from low quality probe intensity data is assessed using a combination of real and synthetic artifacts with PCR follow-up confirmation and the affycomp spike in data. The caCORRECT tool can be accessed at the website: http://cacorrect.bme.gatech.edu.ResultsWe demonstrate that (1) caCORRECT's artifact-aware normalization avoids the undesirable global data warping that happens when any damaged chips are processed without caCORRECT; (2) When used upstream of RMA, PLIER, or MAS5.0, the data imputation of caCORRECT generally improves the accuracy of microarray gene expression in the presence of artifacts more than using Harshlighting or not using any quality control; (3) Biomarkers selected from artifactual microarray data which have undergone the quality control procedures of caCORRECT are more likely to be reliable, as shown by both spike in and PCR validation experiments. Finally, we present a case study of the use of caCORRECT to reliably identify biomarkers for renal cell carcinoma, yielding two diagnostic biomarkers with potential clinical utility, PRKAB1 and NNMT.ConclusionscaCORRECT is shown to improve the accuracy of gene expression, and the reproducibility of experimental results in clinical application. This study suggests that caCORRECT will be useful to clean up possible artifacts in new as well as archived microarray data.

【 授权许可】

Unknown   
© Moffitt et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.