Infrared laser therapy decreases systemic oxidative stress and inflammation in hypercholesterolemic mice with periodontitis

Lipids in Health and Disease

Research

Larissa Trarbach Figueiredo Braga¹  Isadora Martins Ribeiro¹  Silvana dos Santos Meyrelles²  Bianca Prandi Campagnaro³  Edgar Hell Kampke⁴  Maria Eduarda de Souza Barroso⁴ 

[1]Graduate Program in Dental Sciences, Federal University of Espírito Santo (UFES), 29.043-900, Vitória, ES, Brazil

[2]Graduate Program in Dental Sciences, Federal University of Espírito Santo (UFES), 29.043-900, Vitória, ES, Brazil

[3]Graduate Program in Physiological Sciences, Federal University of Espírito Santo (UFES), 29.043-900, Vitória, ES, Brazil

[4]Graduate Program in Pharmaceutical Sciences, Vila Velha University (UVV), 29102-920, Vila Velha, ES, Brazil

[5]Graduate Program in Physiological Sciences, Federal University of Espírito Santo (UFES), 29.043-900, Vitória, ES, Brazil

关键词: Photobiomodulation;    Low-level light therapy;    ApoE knockout;    Periodontal disease;    Hypercholesterolemia;    Oxidative stress;   

DOI  :  10.1186/s12944-023-01934-9

 received in 2023-07-31, accepted in 2023-10-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundNear-infrared irradiation photobiomodulation (NIR-PBM) has been successfully used in periodontal treatment as an adjuvant tool to locally improve cell function and regeneration. Although the relationship between periodontitis and systemic disease constitutes an important aspect of periodontal clinical research, the systemic effects of NIR-PBM in periodontitis are not well known. In this study, we aimed to investigate the effects of NIR-PBM on systemic oxidative stress and inflammation in an apolipoprotein E (ApoE) knockout mouse model of periodontal disease (PD).MethodsWe evaluated alveolar bone loss by measuring the distance from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC), reactive oxygen species (ROS) production in blood cells, inflammatory activity, plasma cholesterol levels, and lipid peroxidation levels in three experimental groups: (1) ApoEC, control group without intervention; (2) ApoEP, first molar ligation-induced periodontitis for 4 weeks; and (3) ApoEP + PBM, exposed to 808 nm continuous wave, ø ~ 3 mm2, 100 mW, 60 s of NIR-PBM for 7 consecutive days after 4 weeks of periodontitis. At the end of the experimental protocols, ApoEP mice presented significantly increased alveolar bone loss, ROS production, inflammatory activity, plasma cholesterol, and lipid peroxidation levels compared to the ApoEC group (P < 0.05). NIR-PBM for 7 days in the ApoEP + PBM mice significantly decreased systemic ROS production, inflammatory response, plasma cholesterol, and lipid peroxidation levels, similar to those found in the ApoEC group (P > 0.05). However, it was not capable of preventing alveolar bone loss (P > 0.05 compared to ApoEP mice).ConclusionA 7-day treatment with NIR-PBM effectively reduces systemic oxidative stress and inflammatory parameters in hypercholesterolemic mice with PD. However, more studies with longer evaluation times are needed to confirm the systemic effects of locally applied NIR-PBM on PD associated with hypercholesterolemia.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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