The plasma proteomic signature as a strategic tool for early diagnosis of acute coronary syndrome

Proteome Science

Research

Luis R Padial¹  José Moreu²  Fernando Vivanco³  María G Barderas⁴  Sergio Alonso-Orgaz⁴  Laura Mourino-Alvarez⁴  Carlos M Laborde⁴ 

[1] Department of Cardiology, Hospital Virgen de la Salud, Toledo, Spain;Department of Hemodynamic, Hospital Virgen de la Salud, SESCAM, Toledo, Spain;Department of Immunology, IIS-Fundación Jiménez Diaz, Madrid, Spain;Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain;Laboratory of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain;

关键词: Acute coronary syndrome;    Protein;    Biomarker;    Two-dimensional fluorescence difference in gel electrophoresis;   

DOI  :  10.1186/1477-5956-12-43

 received in 2014-04-01, accepted in 2014-06-29,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAcute coronary syndrome is the major cause of death in developed countries. Despite its high prevalence, there is still a strong need for new biomarkers which permit faster and more accurate diagnostics and new therapeutic drugs. The basis for this challenge lay in improving our understanding of the whole atherosclerotic process from atherogenesis to atherothrombosis. In this study, we conducted two different proteomic analyses of peripheral blood plasma from non-ST elevation acute coronary syndrome and ST elevation acute coronary syndrome patients vs healthy controls.ResultsTwo-dimensional Fluorescence Difference in Gel Electrophoresis and mass spectrometry permitted the identification of 31 proteins with statistical differences (p < 0.05) between experimental groups. Additionally, validation by Western blot and Selected Reaction Monitoring permitted us to confirm the identification of a different and characteristic plasma proteomic signature for NSTEACS and STEACS patients.ConclusionsWe purpose the severity of hypoxia as the cornerstone for explaining the differences observed between both groups.

【 授权许可】

Unknown   
© Laborde et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.