Determine the quality of human embryonic stem colonies with laser light scattering patterns

Biological Procedures Online

Research

Eric Y-T Chen¹  Wei-Chun Chin¹  Chi-Shuo Chen¹  E Daniel Hirleman²  Matthew Biasca²  Catherine Le³ 

[1] Bioengineering, School of Engineering, University of California, Merced, CA, USA;School of Engineering, University of California, Merced, CA, USA;School of Natural Sciences, University of California, Merced, CA, USA;

关键词: Light-scattering;    Human embryonic stem cell;    Pluripotency;    Label-free detection;   

DOI  :  10.1186/1480-9222-15-2

 received in 2012-08-23, accepted in 2013-01-05,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundWith the prompt developments of regenerative medicine, the potential clinical applications of human embryonic stem cells have attracted intense attention. However, the labor-intensive and complex manual cell selection processes required during embryonic stem cell culturing have seriously limited large-scale production and broad applications. Thus, availability of a label-free, non-invasive platform to replace the current cumbersome manual selection has become a critical need.ResultsA non-invasive, label-free, and time-efficient optical platform for determining the quality of human embryonic stem cell colonies was developed by analyzing the scattering signals from those stem cell colonies. Additionally, confocal microscopy revealed that the cell colony morphology and surface structures were correlated with the resulting characteristic light scattering patterns. Standard immunostaining assay (Oct-4) was also utilized to validate the quality-determination from this light scattering protocol. The platform developed here can therefore provide identification accuracy of up to 87% for colony determination.ConclusionsOur study here demonstrated that light scattering patterns can serve as a feasible alternative approach to replace conventional manual selection for human embryonic stem cell cultures.

【 授权许可】

Unknown   
© Chen et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.