Genome-wide analysis of the auxin/indoleacetic acid (Aux/IAA) gene family in allotetraploid rapeseed (Brassica napus L.)

BMC Plant Biology

Research Article

Bo Wang¹  Qinghua Zhang¹  Haitao Li¹  Jing Wang¹  Kede Liu¹  Graham J. King² 

[1] National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China;Southern Cross Plant Science, Southern Cross University, 2480, Lismore, NSW, Australia;

关键词: Brassica napus;    Aux/IAA;    Chromosome distribution;    Gene duplication;    Expression pattern;    Auxin response;   

DOI  :  10.1186/s12870-017-1165-5

 received in 2017-04-23, accepted in 2017-11-08,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAuxin/Indoleacetic acid (Aux/IAA) genes participate in the auxin signaling pathway and play key roles in plant growth and development. Although the Aux/IAA gene family has been identified in many plants, within allotetraploid Brassica napus little is known.ResultsIn this study, a total of 119 Aux/IAA genes were found in the genome of B. napus. They were distributed non-randomly across all 19 chromosomes and other non-anchored random scaffolds, with a symmetric distribution in the A and C subgenomes. Evolutionary and comparative analysis revealed that 111 (94.1%) B. napus Aux/IAA genes were multiplied due to ancestral Brassica genome triplication and recent allotetraploidy from B. rapa and B. oleracea. Phylogenetic analysis indicated seven subgroups containing 29 orthologous gene sets and two Brassica-specific gene sets. Structures of genes and proteins varied across different genes but were conserved among homologous genes in B. napus. Furthermore, analysis of transcriptional profiles revealed that the expression patterns of Aux/IAA genes in B. napus were tissue dependent. Auxin-responsive elements tend to be distributed in the proximal region of promoters, and are significantly associated with early exogenous auxin up-regulation.ConclusionsMembers of the Aux/IAA gene family were identified and analyzed comprehensively in the allotetraploid B. napus genome. This analysis provides a deeper understanding of diversification of the Aux/IAA gene family and will facilitate further dissection of Aux/IAA gene function in B. napus.

【 授权许可】

CC BY   
© The Author(s). 2017