Nicotine stimulates collagen type I expression in lung via α7 nicotinic acetylcholine receptors

Respiratory Research

Research

Tanmay S. Panchabhai¹  Jeffrey D. Ritzenthaler¹  Edilson Torres-González¹  Glenn W. Vicary²  Jesse Roman³ 

[1] Department of Medicine, Division of Pulmonary, Critical Care and Sleep Disorders, University of Louisville School of Medicine, 550 South Jackson Street (3rd floor), Louisville, KY, USA;Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY, USA;Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY, USA;Department of Medicine, Division of Pulmonary, Critical Care and Sleep Disorders, University of Louisville School of Medicine, 550 South Jackson Street (3rd floor), Louisville, KY, USA;Louisville Robley Rex Veterans Affairs Medical Center, Louisville, KY, USA;

关键词: Tobacco;    Nicotine;    Lung;    Matrix;    Integrin;    Tissue remodeling;    Collagen;   

DOI  :  10.1186/s12931-017-0596-8

 received in 2016-12-11, accepted in 2017-05-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTobacco-related chronic lung diseases are characterized by alterations in lung architecture leading to decreased lung function. Knowledge of the exact mechanisms involved in tobacco-induced tissue remodeling and inflammation remains incomplete. We hypothesize that nicotine stimulates the expression of extracellular matrix proteins, leading to relative changes in lung matrix composition, which may affect immune cells entering the lung after injury.MethodsPulmonary fibroblasts from wildtype and α7 nicotinic acetylcholine receptor knockout (α7KO) mice were exposed to nicotine and examined for collagen type 1 mRNA and protein expression. Testing the potential role on immune cell function, pulmonary fibroblasts were retained in culture for 120 h. The fibroblasts were eliminated by osmotic lysis and the remaining matrix-coated dishes were washed thoroughly. U937 cells were incubated on the matrix-coated dishes for 24 h followed by evaluation of IL-1β gene expression. Wildtype or α7KO C57BL/6 mice (female, 8–12 weeks) were fed normal diet and exposed to nicotine in their drinking water (100 μg/ml) for 8-12weeks. Lungs were processed for mRNA, protein, and histology. Statistical significance was determined at p ≤ .05 by two-tailed test or 2-way ANOVA with Bonferroni posttest.ResultsWe found that nicotine stimulated collagen type I mRNA and protein expression in a dose-dependent manner and up to 72 h in primary lung fibroblasts. The stimulatory effect of nicotine was inhibited in α7KO primary lung fibroblasts. Testing the potential role of these events on immune cell function, U937 monocytic cells were cultured atop matrices derived from nicotine-treated lung fibroblasts. These cells expressed more IL-1β than those cultured atop matrices derived from untreated fibroblasts, and antibodies against the α2β1 collagen integrin receptor inhibited the effect. Nicotine also stimulated fibroblast proliferation via MEK-1/ERK, unveiling a potentially amplifying pathway. In vivo, nicotine increased collagen type I expression was detected in wildtype, but not in α7KO mice. Wildtype mice showed increased collagen staining in lung, primarily around the airways.ConclusionsThese observations suggest that nicotine stimulates fibroblast proliferation and their expression of collagen type I through α7 nAChRs, thereby altering the relative composition of the lung matrix without impacting the overall lung architecture; this may influence inflammatory responses after injury.

【 授权许可】

CC BY   
© The Author(s). 2017