Supplementation of a western diet with golden kiwifruits (Actinidia chinensis var.'Hort 16A':) effects on biomarkers of oxidation damage and antioxidant protection

Nutrition Journal

Research

Asgeir Brevik¹  Tirill Medin²  Rune Blomhoff²  Aud Jørgenesen²  Andrew R Collins²  Anette Karlsen²  Asim K Duttaroy²  Turid Veggan²  Anita Piasek³  Isabel Gaivão⁴  Johanna Elilasson⁵ 

[1] Department of Chemical Toxicology, Division of Environmental Medicine, Norwegian Institute of Public Health, P.O.Box 4404, N-0403, Nydalen, OSLO, Norway;Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316, Oslo, Norway;Gdansk University of Technology, Gdansk, Poland;Genetic and Biotechnology Department, University of Trás-os-Montes and Alto Douro, Quinta de Prados, 5001, Vila Real Codex, Portugal;TATAA Biocenter AB, Odinsgatan 28, 411 03, Göteborg, Sweden;

关键词: Comet Assay;    Nucleotide Excision Repair;    Base Excision Repair;    Plasma Vitamin;    Total Ascorbic Acid;   

DOI  :  10.1186/1475-2891-10-54

 received in 2010-12-10, accepted in 2011-05-18,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe health positive effects of diets high in fruits and vegetables are generally not replicated in supplementation trials with isolated antioxidants and vitamins, and as a consequence the emphasis of chronic disease prevention has shifted to whole foods and whole food products.MethodsWe carried out a human intervention trial with the golden kiwifruit, Actinidia chinensis, measuring markers of antioxidant status, DNA stability, plasma lipids, and platelet aggregation. Our hypothesis was that supplementation of a normal diet with kiwifruits would have an effect on biomarkers of oxidative status. Healthy volunteers supplemented a normal diet with either one or two golden kiwifruits per day in a cross-over study lasting 2 × 4 weeks. Plasma levels of vitamin C, and carotenoids, and the ferric reducing activity of plasma (FRAP) were measured. Malondialdehyde was assessed as a biomarker of lipid oxidation. Effects on DNA damage in circulating lymphocytes were estimated using the comet assay with enzyme modification to measure specific lesions; another modification allowed estimation of DNA repair.ResultsPlasma vitamin C increased after supplementation as did resistance towards H2O2-induced DNA damage. Purine oxidation in lymphocyte DNA decreased significantly after one kiwifruit per day, pyrimidine oxidation decreased after two fruits per day. Neither DNA base excision nor nucleotide excision repair was influenced by kiwifruit consumption. Malondialdehyde was not affected, but plasma triglycerides decreased. Whole blood platelet aggregation was decreased by kiwifruit supplementation.ConclusionGolden kiwifruit consumption strengthens resistance towards endogenous oxidative damage.

【 授权许可】

CC BY   
© Brevik et al; licensee BioMed Central Ltd. 2011