Fatty acids do not pay the toll: effect of SFA and PUFA on human adipose tissue and mature adipocytes inflammation

Lipids in Health and Disease

Research

Manoj Kumar Gunasekaran¹  Ravi Kumar Murumalla¹  Régis Roche¹  Maya Césari¹  Franck Festy¹  Karima Bencharif¹  Jibesh Kumar Padhan¹  Lydie Gence¹  Laurence Hoareau¹ 

[1] GEICO (Study Group on Chronic Inflammation and Obesity), Platform ‘Cyclotron Reunion Ocean Indien’ CYROI, 2, rue Maxime Rivière, 97 490, Sainte-Clotilde, Reunion Island, France;

关键词: FFA;    Human adipose tissue;    Inflammation;    TLR;   

DOI  :  10.1186/1476-511X-11-175

 received in 2012-05-31, accepted in 2012-12-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundOn the basis that high fat diet induces inflammation in adipose tissue, we wanted to test the effect of dietary saturated and polysunsaturated fatty acids on human adipose tissue and adipocytes inflammation. Moreover we wanted to determine if TLR2 and TLR4 are involved in this pathway.MethodsHuman adipose tissue and adipocytes primary cultures were treated with endotoxin-free BSA conjugated with SFA (lauric acid and palmitic acid - LA and PA) and PUFA (eicosapentaeneic acid, docosahexaenoic acid and oleic acid - EPA, DHA and OA) with or without LPS. Cytokines were then assayed by ELISA (TNF-alpha, IL-6 and MCP-1). In order to determine if TLR2 and TLR4 are activated by fatty acid (FA), we used HEK-Blue cells transfected by genes from TLR2 or TLR4 pathways associated with secreted alkaline phosphatase reporter gene.ResultsNone of the FA tested in HEK-Blue cells were able to activate TLR2 or TLR4, which is concordant with the fact that after FA treatment, adipose tissue and adipocytes cytokines levels remain the same as controls. However, all the PUFA tested: DHA, EPA and to a lesser extent OA down-regulated TNF-alpha, IL-6 and MCP-1 secretion in human adipose tissue and adipocytes cultures.ConclusionsThis study first confirms that FA do not activate TLR2 and TLR4. Moreover by using endotoxin-free BSA, both SFA and PUFA tested were not proinflammatory in human adipose tissue and adipocytes model. More interestingly we showed that some PUFA exert an anti-inflammatory action in human adipose tissue and adipocytes model. These results are important since they clarify the relationship between dietary fatty acids and inflammation linked to obesity.

【 授权许可】

Unknown   
© Murumalla et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.