Impact of visceral fat on gene expression profile in peripheral blood cells in obese Japanese subjects

Cardiovascular Diabetology

Original Investigation

Seiji Nakamura¹  Ken-ichi Matsubara¹  Yuji Matsuzawa²  Yuya Yamada²  Koji Yamamoto²  Shigeki Masuda³  Yoshimitsu Tanaka³  Hirofumi Nagao³  Yuya Fujishima³  Iichiro Shimomura³  Hitoshi Nishizawa³  Shiro Fukuda³  Yoshinari Obata³  Masaya Yamaoka³  Tohru Funahashi⁴  Norikazu Maeda⁴  Shunbun Kita⁴ 

[1] DNA Chip Research Inc., 1-15-1 Kaigan, Suzuebaydium 5F, Minato-ku, 105-0022, Tokyo, Japan;Department of Endocrinology and Metabolism, Sumitomo Hospital, 5-3-20, Nakanoshima, Kita-ku, 530-0005, Osaka, Osaka, Japan;Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, 565-0871, Suita, Osaka, Japan;Department of Metabolism and Atherosclerosis, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, 565-0871, Suita, Osaka, Japan;

关键词: Obesity;    Visceral fat;    Subcutaneous fat;    Fat distribution;    Gene expression;    Microarray;    Metabolic syndrome;    Diabetes;    Adiponectin;    KLF;   

DOI  :  10.1186/s12933-016-0479-1

 received in 2016-08-26, accepted in 2016-11-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundVisceral fat plays a central role in the development of metabolic syndrome and atherosclerotic cardiovascular diseases. The association of visceral fat accumulation with cardio-metabolic diseases has been reported, but the impact of visceral fat on the gene expression profile in peripheral blood cells remains to be determined. The aim of this study was to determine the effects of visceral fat area (VFA) and subcutaneous fat area (SFA) on the gene expression profile in peripheral blood cells of obese subjects.MethodsAll 17 enrolled subjects were hospitalized to receive diet therapy for obesity (defined as body mass index, BMI, greater than 25 kg/m2). VFA and SFA were measured at the umbilical level by computed tomography (CT). Blood samples were subjected to gene expression profile analysis by using SurePrint G3 Human GE Microarray 8 × 60 k ver. 2.0. The correlation between various clinical parameters, including VFA and SFA, and peripheral blood gene expression levels was analyzed.ResultsAmong the 17 subjects, 12 had normal glucose tolerance or borderline diabetes, and 5 were diagnosed with type 2 diabetes without medications [glycated hemoglobin (HbA1c); 6.3 ± 1.3%]. The mean BMI, VFA, and SFA were 30.0 ± 5.5 kg/m2, 177 ± 67 and 245 ± 131 cm2, respectively. Interestingly, VFA altered the expression of 1354 genes, including up-regulation of 307 and down-regulation of 1047, under the statistical environment that the parametric false discovery rate (FDR) was less than 0.1. However, no significant effects were noted for SFA or BMI. Gene ontology analysis showed higher prevalence of VFA-associated genes than that of SFA-associated genes, among the genes associated with inflammation, oxidative stress, immune response, lipid metabolism, and glucose metabolism.ConclusionsAccumulation of visceral fat, but not subcutaneous fat, has a significant impact on the gene expression profile in peripheral blood cells in obese Japanese subjects.

【 授权许可】

CC BY   
© The Author(s) 2016