Overexpression of miR-126 sensitizes osteosarcoma cells to apoptosis induced by epigallocatechin-3-gallate

World Journal of Surgical Oncology

Research

Xiaojie He¹  Liangdong Jiang²  Cheng Tao²  Aiyong He² 

[1] Children’s Medical Center, The Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Road, 410011, Changsha, Hunan, P.R. China;Department of Orthopedics, The Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Road, 410011, Changsha, Hunan, P.R. China;

关键词: Osteosarcoma;    miR-126;    Epigallocatechin-3-gallate;    Sensitization;    Apoptosis;   

DOI  :  10.1186/1477-7819-12-383

 received in 2014-09-17, accepted in 2014-12-03,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundmiR-126 plays an important role in the proliferation, invasion, migration, and chemotherapeutics resistance in cancer. Epigallocatechin-3-gallate (EGCG), as the major polyphenolic constituent present in green tea, is a promising anticancer agent. However, the role of miR-126 in EGCG anticancer remains unclear. Here, we investigated the effects of miR-126 and EGCG on cell viability, apoptosis, cell cycle distribution of osteosarcoma cells and the sensitization of miR-126 on osteosarcoma cells to EGCG.MethodsThe cell viability, apoptosis and cycle distribution were analyzed using MTT assay and flow cytometry.ResultsOur results showed that EGCG (0.025, 0.05, 0.1, 0.2 g/L) suppresses proliferation of osteosarcoma MG63 and U2OS cells in a concentration-dependent and time-dependent manner and the inhibitory effects of 0.05 g/L EGCG on U2OS cells were roughly equivalent to 20 μM cisplatin (DDP); miR-126 could promote apoptosis and inhibit proliferation in U2OS cells but without significant effects on cell cycle G1 phase arrest; EGCG suppressed proliferation of U2OS cells through induction of cell cycle G1 arrest and apoptotic death; overexpression of miR-126 enhanced the inhibitory effects of EGCG on proliferation in U2OS cells via promotion of apoptosis.ConclusionsOur results demonstrate that enhanced expression of miR-126 increased the sensitivity of osteosarcoma cells to EGCG through induction of apoptosis.

【 授权许可】

Unknown   
© Jiang et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.