Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticus NS8 from Mongolian fermented koumiss

BMC Microbiology

Research Article

Ming Liu¹  Feng Jin²  Tao Wang²  Xu Hu²  Xingwei Zhang³  Houfeng Zheng³  Jingjing Rong³  Li Wang³ 

[1] Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China;University of the Chinese Academy of Sciences, Beijing, China;Institute of Psychology, Chinese Academy of Sciences, Beijing, China;The Affiliated Hospital, School of Medicine, Hangzhou Normal University, Hangzhou, China;

关键词: L. helveticus;    Anti-inflammatory effect;    S-layer protein;    Koumiss;   

DOI  :  10.1186/s12866-015-0525-2

 received in 2014-11-13, accepted in 2015-09-21,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundKoumiss is a traditionally fermented mare’s milk described with health-promoting potentials for decades. However, only a few studies focused on the probiotic strains isolated from koumiss. In this study, we collected koumiss samples from Inner Mongolian pasturing area of China and selected a promising strain of Lactobacillus helveticus, isolate NS8, based on the survival abilities in gastrointestinal tract (GIT) and adhesion to intestinal endothelial cells in vitro. As the ability to positively modulate host immune response is a feature of increasing importance in measuring the probiotic potential of a bacterial strain, our study mainly focus on the immunomodulatory properties of L. helveticus NS8 by using in vivo and ex vivo analyses.ResultsL. helveticus NS8 was identified by molecular-typing methods, both at genus and species levels. As a typical food niche-specific bacteria, NS8 showed a moderate survival ability in GIT environment in vitro. However, an excellent binding capacity to the human intestinal epithelial cells, along with significant autoaggregation and cell-surface hydrophobicity was observed. Additionally, the presence of S-layer protein was responsible for the cell surface properties of this strain. NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis. In the meantime, co-culture with NS8 induced an increased level of secretion of anti-inflammatory cytokine IL-10 in peripheral blood mono-nuclear cells (PBMCs). Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10. Specially, adding of the purified S-layer protein didn’t influence the production of IL-10. The specific ligand-host receptor interactions on the NS8 specific immune responses need to be learned further.ConclusionIn summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.

【 授权许可】

CC BY   
© Rong et al. 2015