Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds

Microbial Cell Factories

Review

Guillermo Gosset¹  Juan A Martnez¹  Alberto Rodriguez¹  Adelfo Escalante¹  Noem Flores¹  Francisco Bolivar¹ 

[1] Departamento de Ingeniera Celular y Biocatlisis, Instituto de Biotecnologa Universidad Nacional Autonoma de Mexico (UNAM), Avenida Universidad 2001, Col. Chamilpa, Cuernavaca, 62210, Morelos, Mexico;

关键词: Aromatic compounds;    Escherichia coli;    Metabolic engineering;    Systems biotechnology;    Synthetic biology;    Shikimate pathway;    Phenylalanine;    Tyrosine;    Tryptophan;   

DOI  :  10.1186/s12934-014-0126-z

 received in 2014-05-30, accepted in 2014-08-17,  发布年份 2014

来源: Springer

【 摘 要 】

The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids and primary metabolites, the artificial channelling of building blocks from central metabolism towards the aromatic amino acid pathway is complicated to achieve in an efficient manner. The length and complex regulation of this pathway have progressively called for the employment of more integral approaches, promoting the merge of complementary tools and techniques in order to surpass metabolic and regulatory bottlenecks. As a result, relevant insights on the subject have been obtained during the last years, especially with genetically modified strains of Escherichia coli. By combining metabolic engineering strategies with developments in synthetic biology, systems biology and bioprocess engineering, notable advances were achieved regarding the generation, characterization and optimization of E. coli strains for the overproduction of aromatic amino acids, some of their precursors and related compounds. In this paper we review and compare recent successful reports dealing with the modification of metabolic traits to attain these objectives.

【 授权许可】

Unknown   
© Rodriguez et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.