Parallel action of AtDRB2 and RdDM in the control of transposable element expression

BMC Plant Biology

Research Article

Cyril Charbonel¹  Viviane Jean¹  Jean-Marc Deragon¹  Julio Saez-Vásquez¹  Julie Descombin¹  Claire Picart¹  Cécile Bousquet-Antonelli¹  Marion Clavel²  Thierry Pélissier³ 

[1] Université de Perpignan Via Domitia, LGDP UMR CNRS-UPVD 5096, 58 Av. Paul Alduy, 66860, Perpignan Cedex, France;CNRS UMR5096 LGDP, Perpignan Cedex, France;Université de Perpignan Via Domitia, LGDP UMR CNRS-UPVD 5096, 58 Av. Paul Alduy, 66860, Perpignan Cedex, France;CNRS UMR5096 LGDP, Perpignan Cedex, France;Present address: IBMP, UPR 2357, 12, rue du général Zimmer, 67084, Strasbourg cedex, France;Université de Perpignan Via Domitia, LGDP UMR CNRS-UPVD 5096, 58 Av. Paul Alduy, 66860, Perpignan Cedex, France;CNRS UMR5096 LGDP, Perpignan Cedex, France;Present address: UMR6293 CNRS - INSERM U1103 – GreD, Clermont Université, 24 avenue des Landais, B.P. 80026, 63171, Aubière Cedex, France;

关键词: RNAi;    siRNA;    Double-stranded RNA binding protein;    Epigenetics;    Chromatin;    Arabidopsis;   

DOI  :  10.1186/s12870-015-0455-z

 received in 2015-01-22, accepted in 2015-02-13,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn plants and animals, a large number of double-stranded RNA binding proteins (DRBs) have been shown to act as non-catalytic cofactors of DICERs and to participate in the biogenesis of small RNAs involved in RNA silencing. We have previously shown that the loss of Arabidopsis thaliana’s DRB2 protein results in a significant increase in the population of RNA polymerase IV (p4) dependent siRNAs, which are involved in the RNA-directed DNA methylation (RdDM) process.ResultsSurprisingly, despite this observation, we show in this work that DRB2 is part of a high molecular weight complex that does not involve RdDM actors but several chromatin regulator proteins, such as MSI4, PRMT4B and HDA19. We show that DRB2 can bind transposable element (TE) transcripts in vivo but that drb2 mutants do not have a significant variation in TE DNA methylation.ConclusionWe propose that DRB2 is part of a repressive epigenetic regulator complex involved in a negative feedback loop, adjusting epigenetic state to transcription level at TE loci, in parallel of the RdDM pathway. Loss of DRB2 would mainly result in an increased production of TE transcripts, readily converted in p4-siRNAs by the RdDM machinery.

【 授权许可】

Unknown   
© Clavel et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.