Effect of fish oil intake on glucose levels in rat prefrontal cortex, as measured by microdialysis

Lipids in Health and Disease

Research

Mônica M Telles¹  Isy F de Sousa¹  Claúdia MO Nascimento¹  Valter T Boldarine¹  Lila M Oyama¹  Iracema S Andrade¹  Eliane B Ribeiro¹  Adriana P de Souza¹ 

[1] Departamento de Fisiologia, Universidade Federal de São Paulo (Unifesp), Rua Botucatu, n° 862 – 2° andar, Vila Clementino, 04023-062, São Paulo, SP, Brazil;

关键词: Food intake;    Obesity;    Long-chain omega-3 fatty acids;    Brain microdialysis;   

DOI  :  10.1186/1476-511X-12-188

 received in 2013-08-08, accepted in 2013-12-21,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundBrain glucose sensing may contribute to energy homeostasis control. The prefrontal cortex (PFC) participates in the hedonic component of feeding control. As high-fat diets may disrupt energy homeostasis, we evaluated in male Wistar rats whether intake of high-fat fish-oil diet modified cortical glucose extracellular levels and the feeding induced by intracerebroventricular glucose or PFC glucoprivation.MethodsGlucose levels in PFC microdialysates were measured before and after a 30-min meal. Food intake was measured in animals receiving intracerebroventricular glucose followed, 30-min. later, by 2-deoxy-D-glucose injected into the PFC.ResultsThe fish-oil group showed normal body weight and serum insulin while fat pads weight and glucose levels were increased. Baseline PFC glucose and 30-min. carbohydrates intake were similar between the groups. Feeding-induced PFC glucose levels increased earlier and more pronouncedly in fish-oil than in control rats. Intracerebroventricular glucose inhibited feeding consistently in the control but not in the fish-oil group. Local PFC glucoprivation with 2-DG attenuated glucose-induced hypophagia.ConclusionsThe present experiments have shown that, following food intake, more glucose reached the prefrontal cortex of the rats fed the high-fat fish-oil diet than of the rats fed the control diet. However, when administered directly into the lateral cerebral ventricle, glucose was able to consistently inhibit feeding only in the control rats. The findings indicate that, an impairment of glucose transport into the brain does not contribute to the disturbances induced by the high-fat fish-oil feeding.

【 授权许可】

Unknown   
© de Sousa et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.