Cathepsin B: a potential prognostic marker for inflammatory breast cancer

Journal of Translational Medicine

Research

Mohamed El-Shinawi¹  Hussein M Khaled²  Mohamed A Nouh³  Bonnie F Sloane⁴  Dora Cavallo-Medved⁵  Mohamed A Shaalan⁶  Mona M Mohamed⁷ 

[1] Department of General Surgery, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt;Department of Medical Oncology, National Cancer Institute, Cairo University, 12613, Giza, Egypt;Department of Pathology, National Cancer Institute, Cairo University, 12613, Giza, Egypt;Department of Pharmacology, Wayne State University, 48201, Detroit, MI, USA;Barbara Ann Karmanos Cancer Institute, Wayne State University, 48201, Detroit, MI, USA;Department of Pharmacology, Wayne State University, 48201, Detroit, MI, USA;Department of Biological Sciences, University of Windsor, N9B 3P4, Windsor, ON, Canada;Department of Surgery, National Cancer institute, Cairo University, 12613, Giza, Egypt;Department of Zoology, Faculty of Science, Cairo University, 12613, Giza, Egypt;

关键词: Inflammatory Breast Cancer;    Tumor Embolus;    Positive Lymph Node Metastasis;    Cancer Cell Motility;    Inflammatory Breast Cancer Patient;   

DOI  :  10.1186/1479-5876-9-1

 received in 2010-08-21, accepted in 2011-01-03,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundInflammatory breast cancer (IBC) is the most aggressive form of breast cancer. In non-IBC, the cysteine protease cathepsin B (CTSB) is known to be involved in cancer progression and invasion; however, very little is known about its role in IBC.MethodsIn this study, we enrolled 23 IBC and 27 non-IBC patients. All patient tissues used for analysis were from untreated patients. Using immunohistochemistry and immunoblotting, we assessed the levels of expression of CTSB in IBC versus non-IBC patient tissues. Previously, we found that CTSB is localized to caveolar membrane microdomains in cancer cell lines including IBC, and therefore, we also examined the expression of caveolin-1 (cav-1), a structural protein of caveolae in IBC versus non-IBC tissues. In addition, we tested the correlation between the expression of CTSB and cav-1 and the number of positive metastatic lymph nodes in both patient groups.ResultsOur results revealed that CTSB and cav-1 were overexpressed in IBC as compared to non-IBC tissues. Moreover, there was a significant positive correlation between the expression of CTSB and the number of positive metastatic lymph nodes in IBC.ConclusionsCTSB may initiate proteolytic pathways crucial for IBC invasion. Thus, our data demonstrate that CTSB may be a potential prognostic marker for lymph node metastasis in IBC.

【 授权许可】

Unknown   
© Nouh et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.