No evidence of association between human cytomegalovirus infection and papillary thyroid cancer

World Journal of Surgical Oncology

Research

Tung-Sun Huang¹  Shih-Ping Cheng²  Jie-Jen Lee² 

[1] Department of Surgery, Mackay Medical College and Mackay Memorial Hospital, No. 92, Sec 2, Chung-Shan North Road, 10449, Taipei, Taiwan;Department of Surgery, Mackay Medical College and Mackay Memorial Hospital, No. 92, Sec 2, Chung-Shan North Road, 10449, Taipei, Taiwan;Mackay Junior College of Medicine, Nursing, and Management, No. 92, Sheng-Ching Road, 11260, Peitou, Taipei, Taiwan;Department of Pharmacology and Graduate Institute of Medical Sciences, Taipei Medical University, No. 250, Wu-Hsing Street, 11031, Taipei, Taiwan;

关键词: Cytomegalovirus;    BRAF;    Papillary thyroid cancer;   

DOI  :  10.1186/1477-7819-12-41

 received in 2013-10-17, accepted in 2014-02-12,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHuman cytomegalovirus (CMV) has been detected in the thyroid gland and thyroid tumors. CMV infection may activate the mitogen-activated protein kinase pathway, of which aberrant activation is frequently associated with BRAF mutation in papillary thyroid cancer.MethodsA total of 45 paired tumorous and adjacent non-neoplastic tissue samples, including 5 follicular adenoma and 40 papillary thyroid cancer, were obtained during thyroidectomy. BRAF mutational status was determined using direct sequencing. The presence of CMV DNA was determined using conventional PCR and quantitative real-time PCR. CMV protein in the tissue samples were evaluated with Western blot analysis.ResultsBRAF mutation was identified in the cancerous part of 31 (78%) papillary thyroid cancers. Papillary cancer with BRAF mutation was significantly associated with a larger tumor size (P = 0.045), extrathyroidal invasion (P = 0.012), lymph node metastasis (P = 0.008), and a higher TNM stage (P = 0.044). CMV DNA and protein were not detected in any studied samples.ConclusionsOur results suggest no association between CMV infection and papillary thyroid cancer.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.