Spatiotemporal expression of the serine protease inhibitor, SERPINE2, in the mouse placenta and uterus during the estrous cycle, pregnancy, and lactation

Reproductive Biology and Endocrinology

Research

Edmund I Tsuen Chen¹  Schu-Rern Chern²  Chung-Hao Lu³  Sheng-Hsiang Li⁴ 

[1] Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan;Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan;Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan;Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan;Graduate Institute of Biotechnology, National Taipei University of Technology, Taipei, Taiwan;

关键词: Estrous Cycle;    Mouse Uterus;    Trophoblast Giant Cell;    Pregnant Uterus;    Ectoplacental Cone;   

DOI  :  10.1186/1477-7827-8-127

 received in 2010-06-29, accepted in 2010-10-27,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundSERPINE2, also known as glia-derived nexin or protease nexin-1, belongs to the serine protease inhibitor (SERPIN) superfamily. It is one of the potent serpins that modulates the activity of the plasminogen activator (PA) and was implicated in tissue remodeling. In this study, we investigated the expression patterns of SERPINE2 in the mouse placenta and uterus during the estrous cycle, pregnancy, and lactation.MethodsSERPINE2 was purified from mouse seminal vesicle secretion using liquid chromatography (LC) and identified by LC/tandem mass spectrometry. The antiserum against the SERPINE2 protein was raised in rabbits. To reveal the uterine and placental expression of SERPINE2, tissues at various stages were collected for real-time PCR quantification, Western blotting, and immunohistochemical staining.ResultsSerpine2 mRNA was the major PA inhibitor in the placenta and uterus during the estrous cycle, pregnancy, and lactation, although Serpine1 mRNA had higher expression levels than Serpine2 mRNA in the placenta. Plat seemed to be the major PA in the mouse uterus and placenta. Antiserum against the SERPINE2 protein specifically recognized two forms of SERPINE2 and an extra 75-kDa protein, which was probably a complex of SERPINE2 with a certain protease, from among thousands of protein components in the tissue extract as demonstrated by Western blotting. In the uterus, SERPINE2 was primarily localized in luminal and glandular epithelial cells but it also was detected in circular and longitudinal smooth muscle cells during the estrous cycle and lactation. It was prominently expressed in decidual stroma cells, the metrial gland, and endometrial epithelium of the pregnant uterus. In the placenta, SERPINE2 was expressed in trophoblasts of the labyrinth and spongiotrophoblasts. However, its expression was remarkably reduced in giant cells which existed in the giant cell-decidual junction zone. In contrast, prominent expression of SERPINE2 seemed to be detected on clusters of glycogen cells near the junction zone. In addition, yolk sac membranes also showed high expression of SERPINE2.ConclusionsThese findings indicate that SERPINE2 is a major PA inhibitor in the placenta and uterus during the estrous cycle, pregnancy, and lactation. It may participate in the PA-modulated tissue remodeling process in the mouse placenta and uterus.

【 授权许可】

Unknown   
© Chern et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.