Rough surface Au@Ag core–shell nanoparticles to fabricating high sensitivity SERS immunochromatographic sensors

Journal of Nanobiotechnology

Research

Ze Wu¹  Wei Xiao¹  Qiangqiang Fu¹  Cuize Yao¹  Xiuqing Li¹  Shiting Yu¹  Yong Tang²  Hongwu Liu Liu³  Zhi Luo³  An Liu³ 

[1] Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, 510632, Guangzhou, People’s Republic of China;Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, 510632, Guangzhou, People’s Republic of China;Institute of Biotranslational Medicine, Jinan University, 510632, Guangzhou, People’s Republic of China;Integrated Optics and Biophotonics Laboratory, Department of Electronic Engineering, Jinan University, 510632, Guangzhou, People’s Republic of China;

关键词: Rough surface core–shell Au@Ag nanoparticles;    SERS;    Immunochromatographic sensors;    Haemoglobin;    Cadmium ion;   

DOI  :  10.1186/s12951-015-0142-0

 received in 2015-09-08, accepted in 2015-10-26,  发布年份 2015

来源: Springer

【 摘 要 】

Immunochromatographic sensors (ICSs) are inexpensive, simple, portable, and robust, thus making ICSs commonplace in clinical diagnoses, food testing, and environmental monitoring. However, commonly used gold nanoparticles (AuNPs) ICSs have low sensitivity. Therefore, we developed highly sensitive surface enhanced Raman scattering (SERS) ICSs. To enhance the sensitivity of SERS ICSs, rough surface core–shell Au@Ag nanoparticles (RSAu@AgNPs) were prepared by coating silver on the surface of gold nanoflowers (AuNFs). Then these nanoparticles were used as SERS substrate in the SERS ICSs, after which the SERS ICSs were implemented to detect haemoglobin and heavy metal cadmium ion (Cd2+). The limit of detection (LOD) of the SERS ICSs for detecting haemoglobin was 8 ng/mL, and the linear range of the SERS ICSs was from 31.3 to 2000 ng/mL. The LOD of the SERS ICSs for detecting Cd2+ was 0.05 ng/mL and the linear analysis range was from 0.05 to 25 ng/mL. The cross reactivity of the SERS ICSs was studied and results showed that the SERS ICSs exhibited highly specific for detection of haemoglobin and Cd2+, respectively. The SERS ICSs were then used to detect haemoglobin (spiked in serum and in stool) and Cd2+ (spiked in tap water, river water, and soil leaching water), and the results showed high recovery. These characteristics indicated that SERS ICSs were ideal tools for clinical diagnosis and environmental pollution monitoring.

【 授权许可】

CC BY   
© Fu et al. 2015