Study of TLR3, TLR4 and TLR9 in breast carcinomas and their association with metastasis

BMC Cancer

Research Article

Lucía González¹  Laura Marín¹  Salomé González-Reyes¹  Maria L Lamelas¹  Luis O González²  José M González-Quintana²  José M del Casar³  Francisco J Vizoso³ 

[1] Unidad de Investigación, Fundación Hospital de Jove, Gijón, Spain;Unidad de Investigación, Fundación Hospital de Jove, Gijón, Spain;Servicio de Anatomía Patológica, Fundación Hospital de Jove, Gijón, Spain;Unidad de Investigación, Fundación Hospital de Jove, Gijón, Spain;Servicio de Cirugía General, Fundación Hospital de Jove, Gijón, Spain;

关键词: Breast Cancer;    TLR4 Expression;    Tissue Array;    Bilateral Breast Cancer;    Mononuclear Inflammatory Cell;   

DOI  :  10.1186/1471-2407-10-665

 received in 2010-02-10, accepted in 2010-12-03,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundToll-like receptors (TLRs) have garnered an extraordinary amount of interest in cancer research due to their role in tumor progression. By activating the production of several biological factors, TLRs induce type I interferons and other cytokines, which drive an inflammatory response and activate the adaptive immune system. The aim of this study was to investigate the expression and clinical relevance of TLR3, 4 and 9 in breast cancer.MethodsThe expression levels of TLR3, TLR4 and TLR9 were analyzed on tumors from 74 patients with breast cancer. The analysis was performed by immunohistochemistry.ResultsSamples of carcinomas with recurrence exhibited a significant increase in the mRNA levels of TLR3, TLR4 and TLR9. Tumors showed high expression of TLRs expression levels by cancer cells, especially TLR4 and 9. Nevertheless, a significant percentage of tumors also showed TLR4 expression by mononuclear inflammatory cells (21.6%) and TLR9 expression by fibroblast-like cells (57.5%). Tumors with high TLR3 expression by tumor cell or with high TLR4 expression by mononuclear inflammatory cells were significantly associated with higher probability of metastasis. However, tumours with high TLR9 expression by fibroblast-like cells were associated with low probability of metastasis.ConclusionsThe expression levels of TLR3, TLR4 and TLR9 have clinical interest as indicators of tumor aggressiveness in breast cancer. TLRs may represent therapeutic targets in breast cancer.

【 授权许可】

Unknown   
© González-Reyes et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.