QTL mapping and candidate genes for resistance to Fusarium ear rot and fumonisin contamination in maize

BMC Plant Biology

Research Article

Alessandra Lanubile¹  Valentina Maschietto¹  Adriano Marocco¹  Cinzia Colombi²  Giorgio Pea²  Francesco Strozzi²  Laura Rossini³  Raul Pirona⁴ 

[1] Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122, Piacenza, Italy;Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy;Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy;Department of Agricultural and Environmental Sciences Production, Landscape, Agroenergy, Università degli Studi di Milano, Via Celoria 2, 20133, Milano, Italy;Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy;Institute of Agricultural Biology and Biotechnology, CNR, Via Bassini 15, 20133, Milano, Italy;

关键词: Zea mays;    Fusarium verticillioides;    FB1 contamination;    Genotyping-by-Sequencing;   

DOI  :  10.1186/s12870-017-0970-1

 received in 2016-03-11, accepted in 2017-01-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFusarium verticillioides is a common maize pathogen causing ear rot (FER) and contamination of the grains with the fumonisin B1 (FB1) mycotoxin. Resistance to FER and FB1 contamination are quantitative traits, affected by environmental conditions, and completely resistant maize genotypes to the pathogen are so far unknown. In order to uncover genomic regions associated to reduced FER and FB1 contamination and identify molecular markers for assisted selection, an F2:3 population of 188 progenies was developed crossing CO441 (resistant) and CO354 (susceptible) genotypes. FER severity and FB1 contamination content were evaluated over 2 years and sowing dates (early and late) in ears artificially inoculated with F. verticillioides by the use of either side-needle or toothpick inoculation techniques.ResultsWeather conditions significantly changed in the two phenotyping seasons and FER and FB1 content distribution significantly differed in the F3 progenies according to the year and the sowing time. Significant positive correlations (P < 0.01) were detected between FER and FB1 contamination, ranging from 0.72 to 0.81. A low positive correlation was determined between FB1 contamination and silking time (DTS). A genetic map was generated for the cross, based on 41 microsatellite markers and 342 single nucleotide polymorphisms (SNPs) derived from Genotyping-by-Sequencing (GBS). QTL analyses revealed 15 QTLs for FER, 17 QTLs for FB1 contamination and nine QTLs for DTS. Eight QTLs located on linkage group (LG) 1, 2, 3, 6, 7 and 9 were in common between FER and FB1, making possible the selection of genotypes with both low disease severity and low fumonisin contamination. Moreover, five QTLs on LGs 1, 2, 4, 5 and 9 located close to previously reported QTLs for resistance to other mycotoxigenic fungi. Finally, 24 candidate genes for resistance to F. verticillioides are proposed combining previous transcriptomic data with QTL mapping.ConclusionsThis study identified a set of QTLs and candidate genes that could accelerate breeding for resistance of maize lines showing reduced disease severity and low mycotoxin contamination determined by F. verticillioides.

【 授权许可】

CC BY   
© The Author(s). 2017