An integrated approach of comparative genomics and heritability analysis of pig and human on obesity trait: evidence for candidate genes on human chromosome 2

BMC Genomics

Research Article

Taeheon Lee¹  Tae-Hun Kim²  Kyung-Tai Lee²  Jaemin Kim³  Heebal Kim⁴ 

[1] Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea;Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, 441-706, Suwon, Republic of Korea;Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea;Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea;Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea;C&K Genomics, Seoul National University Research Park, 151-919, SeoulRepublic of Korea;

关键词: Obesity;    Synteny;    Comparative genomics;    Heritability;    Back-fat thickness;    Subscapular skinfold thickness;    Chromosome 2;    Pig;    Human;   

DOI  :  10.1186/1471-2164-13-711

 received in 2012-05-07, accepted in 2012-12-04,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundTraditional candidate gene approach has been widely used for the study of complex diseases including obesity. However, this approach is largely limited by its dependence on existing knowledge of presumed biology of the phenotype under investigation. Our combined strategy of comparative genomics and chromosomal heritability estimate analysis of obesity traits, subscapular skinfold thickness and back-fat thickness in Korean cohorts and pig (Sus scrofa), may overcome the limitations of candidate gene analysis and allow us to better understand genetic predisposition to human obesity.ResultsWe found common genes including FTO, the fat mass and obesity associated gene, identified from significant SNPs by association studies of each trait. These common genes were related to blood pressure and arterial stiffness (P = 1.65E-05) and type 2 diabetes (P = 0.00578). Through the estimation of variance of genetic component (heritability) for each chromosome by SNPs, we observed a significant positive correlation (r = 0.479) between genetic contributions of human and pig to obesity traits. Furthermore, we noted that human chromosome 2 (syntenic to pig chromosomes 3 and 15) was most important in explaining the phenotypic variance for obesity.ConclusionsObesity genetics still awaits further discovery. Navigating syntenic regions suggests obesity candidate genes on chromosome 2 that are previously known to be associated with obesity-related diseases: MRPL33, PARD3B, ERBB4, STK39, and ZNF385B.

【 授权许可】

CC BY   
© Kim et al.; licensee BioMed Central Ltd. 2012