Delta-6-desaturase gene polymorphism is associated with lipoprotein oxidation in vitro

Lipids in Health and Disease

Research

Jaana Renko¹  Tarja Kunnas¹  Tiina Solakivi¹  Seppo T Nikkari²  Terho Lehtimäki³  Olli Jaakkola⁴ 

[1] Department of Medical Biochemistry, University of Tampere Medical School, Tampere, Finland;Department of Medical Biochemistry, University of Tampere Medical School, Tampere, Finland;Fimlab Laboratories, Tampere, Finland;Fimlab Laboratories, Tampere, Finland;Institute of Medical Technology, University of Tampere, Tampere, Finland;

关键词: Fatty Acid Composition;    Arachidonic Acid;    Eicosapentaenoic Acid;    Desaturase Gene;    Docosapentaenoic Acid;   

DOI  :  10.1186/1476-511X-12-80

 received in 2013-03-26, accepted in 2013-05-27,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundOxidative modification of low-density lipoprotein (LDL) is a key event in the oxidation hypothesis of atherogenesis. We have previously shown that HDL does not protect LDL from oxidation in vitro, but is in fact oxidized fastest of all lipoproteins due to its rich polyunsaturated fatty acid (PUFA) composition, which is oxidation promoting. Evidence has accumulated to show that in addition to diet, common polymorphisms in the fatty acid desaturase (FADS) gene cluster have very marked effects on human PUFA status. There is a deletion [T/-] in the promoter region of the Δ6 –desaturase gene (FADS2, rs 3834458), which has a direct inhibitory influence on production of PUFA from linoleic and alpha-linolenic acid. To investigate the possible role of rs 3834458 in lipoprotein modification, oxidation of LDL with HDL2 or HDL3 were analyzed from plasma of 58 free-living individuals.ResultsTotal eicosapentaenoic acid and arachidonic acid were significantly decreased in plasma from the 10 subjects homozygous for the deletion in FADS2 rs 3834458. When the isolated LDL and HDL2 were subjected to Cu2+-induced oxidation, these subjects showed decreased rate of appearance (p = 0.027) and the final concentration of conjugated dienes (p = 0.033) compared to the other genotypes. For oxidation of LDL with HDL3, the final concentration of conjugated dienes was also significantly decreased in subjects with [−/−] compared with [T/T] and [T/-] (p = 0.034).ConclusionWe conclude that FADS2 genotype may play a role in peroxidation susceptibility of lipoproteins.

【 授权许可】

Unknown   
© Solakivi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.