Effects of corticosteroid on the expressions of neuropeptide and cytokine mRNA and on tenocyte viability in lateral epicondylitis

Journal of Inflammation

Research

Young Do Kwon¹  Soo Hong Han²  Dong Eun Shin²  Soon Chul Lee²  Jong Sup Shim³  Hee Jung An⁴  Ji Ye Song⁵ 

[1] Department of Biomedical Science, Graduate School, CHA University, 463-712, Gyeonggi-do, Korea;Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University, 463-712, Gyeonggi-do, Korea;Department of Orthopaedic Surgery, Samsung Medical Center, Sungkyunkwan University, 135-710, Seoul, Korea;Department of Pathology, CHA Bundang Medical Center, CHA University, 463-712, Gyeonggi-do, Korea;Institute for Clinical Research, CHA Bundang Medical Center, CHA University, 463-712, Gyeonggi-do, Korea;

关键词: Lateral epicondylitis;    Corticosteroid;    Neuropeptide;    mRNA;    Tenocyte viability;   

DOI  :  10.1186/1476-9255-9-40

 received in 2012-04-23, accepted in 2012-10-26,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe purpose of this study was to determine the reaction mechanism of corticosteroid by analyzing the expression patterns of neuropeptides (substance P (SP), calcitonin gene related peptide (CGRP)) and of cytokines (interleukin (IL)-1α, tumor growth factor (TGF)-β) after corticosteroid treatment in lateral epicondylitis. In addition, we also investigated whether corticosteroid influenced tenocyte viability.MethodsThe corticosteroid triamcinolone acetonide (TAA) was applied to cultured tenocytes of lateral epicondylitis, and the changes in the mRNA expressions of neuropeptides and cytokines and tenocyte viabilities were analyzed at seven time points. Quantitative real-time polymerase chain reaction and an MTT assay were used.ResultsThe expression of SP mRNA was maximally inhibited by TAA at 24 hours but recovered at 72 hours, and the expressions of CGRP mRNA and IL-1α mRNA were inhibited at 24 and 3 hours, respectively. The expression of TGF-β mRNA was not significant. Tenocyte viability was significantly reduced by TAA at 24 hours.ConclusionsWe postulate that the reaction mechanism predominantly responsible for symptomatic relief after a corticosteroid injection involves the inhibitions of neuropeptides and cytokines, such as, CGRP and IL-1α. However the tenocyte viability was compromised by a corticosteroid.

【 授权许可】

Unknown   
© Han et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.