Raman imaging at biological interfaces: applications in breast cancer diagnosis

Molecular Cancer

Research

Jacek Musial¹  Radzislaw Kordek¹  Jakub Surmacki²  Halina Abramczyk² 

[1] Department of Pathology, Chair of Oncology, Medical University of Lodz, Paderewskiego 4, 93-509, Lodz, Poland;Laboratory of Laser Molecular Spectroscopy, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590, Lodz, Poland;

关键词: Breast cancer biochemical imaging;    Interfacial water;    Raman imaging;    IR spectroscopy;   

DOI  :  10.1186/1476-4598-12-48

 received in 2012-12-19, accepted in 2013-05-22,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundOne of the most important areas of Raman medical diagnostics is identification and characterization of cancerous and noncancerous tissues. The methods based on Raman scattering has shown significant potential for probing human breast tissue to provide valuable information for early diagnosis of breast cancer. A vibrational fingerprint from the biological tissue provides information which can be used to identify, characterize and discriminate structures in breast tissue, both in the normal and cancerous environment.ResultsThe paper reviews recent progress in understanding structure and interactions at biological interfaces of the human tissue by using confocal Raman imaging and IR spectroscopy. The important differences between the noncancerous and cancerous human breast tissues were found in regions characteristic for vibrations of carotenoids, fatty acids, proteins, and interfacial water. Particular attention was paid to the role played by unsaturated fatty acids and their derivatives as well as carotenoids and interfacial water.ConclusionsWe demonstrate that Raman imaging has reached a clinically relevant level in regard to breast cancer diagnosis applications. The results presented in the paper may have serious implications on understanding mechanisms of interactions in living cells under realistically crowded conditions of biological tissue.

【 授权许可】

Unknown   
© Surmacki et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.