Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis

BMC Gastroenterology

Research Article

Stig Purup¹  Lotte Bach Larsen²  Nina Aagaard Poulsen²  Hanne Søndergaard Møller²  Vibeke Andersen³  Flemming Jessen⁴  Jens Christian Møller⁵ 

[1] Department of Animal Science, Aarhus University, 8830, Tjele, Denmark;Department of Food Science, Aarhus University, 8830, Tjele, Denmark;Medical Department, Viborg Regional Hospital, 8800, Viborg, Denmark;Medical Department, SHS Aabenraa, 6200, Aabenraa, Denmark;Institute of Regional Health Services Research, South Danish University, 5000, Odense, Denmark;National Food Institute, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark;Pathological Department, Viborg Regional Hospital, 8800, Viborg, Denmark;

关键词: Inflammatory bowel disease;    Ulcerative colitis;    Colon biopsies;    Candidate markers;    MS-based proteomics;   

DOI  :  10.1186/1471-230X-12-76

 received in 2012-02-16, accepted in 2012-06-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAccurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis.MethodsBiopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots.ResultsA total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein).ConclusionsA distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.

【 授权许可】

CC BY   
© Poulsen et al.; licensee BioMed Central Ltd. 2012