Sphingomyelin synthase overexpression increases cholesterol accumulation and decreases cholesterol secretion in liver cells

Lipids in Health and Disease

Research

Yue Li¹  Manping Wu¹  Tingbo Ding¹  Nianlong Yan¹  Jibin Dong¹ 

[1] School of Pharmacy, Fudan University, Shanghai, People's Republic of China;

关键词: Huh7 Cell;    Cholesterol Efflux;    Reverse Cholesterol Transport;    Cellular Cholesterol Level;    High Density Lipoprotein Receptor;   

DOI  :  10.1186/1476-511X-10-46

 received in 2011-03-01, accepted in 2011-03-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundStudies have shown that plasma high density lipoprotein cholesterol levels are negatively correlated with the development of atherosclerosis, whereas epidemiological studies have also shown that plasma sphingomyelin level is an independent risk factor for atherosclerosis.MethodsTo evaluate the relationship between cellular sphingomyelin level and cholesterol metabolism, we created two cell lines that overexpressed sphingomyelin synthase 1 or 2 (SMS1 or SMS2), using the Tet-off expression system.ResultsWe found that SMS1 or SMS2 overexpression in Huh7 cells, a human hepatoma cell line, significantly increased the levels of intracellular sphingomyelin, cholesterol, and apolipoprotein A-I and decreased levels of apolipoprotein A-I and cholesterol in the cell culture medium, implying a defect in both processes.ConclusionsOur findings indicate that the manipulation of sphingomyelin synthase activity could influence the metabolism of sphingomyelin, cholesterol and apolipoprotein A-I.

【 授权许可】

Unknown   
© Yan et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.