Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the human malaria parasite Plasmodium falciparum

Malaria Journal

Methodology

Catherine J. Merrick¹ 

[1] Centre for Applied Entomology and Parasitology, Faculty of Natural Sciences, Keele University, ST55BG, Keele, Staffordshire, UK;

关键词: Plasmodium;    Malaria;    BrdU;    S-phase;    DNA replication;    Cell cycle;   

DOI  :  10.1186/s12936-015-1014-7

 received in 2015-09-08, accepted in 2015-11-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum, the causative agent of severe human malaria, is an early-diverging protozoan whose lifecycle has many unusual features, including its modes of replication. Research on the Plasmodium cell cycle, which occurs primarily via schizogony instead of canonical binary fission, has been hampered by a lack of tools and markers that can be transferred from cell cycle studies in model organisms. A common tool used to study DNA replication and the cell cycle in human cells is the labelling of newly-replicated DNA with the modified nucleotide bromodeoxyuridine (BrdU), followed by immunofluorescent detection. Plasmodium parasites, however, do not incorporate BrdU because they rely only on de novo synthesis of pyrimidines and do not salvage thymidine analogues like BrdU for conversion into nucleotides.MethodsAnalysis of biochemical pathways in Plasmodium indicated that the absence of the enzyme thymidine kinase (TK) may be the only impediment to BrdU incorporation in this organism. A TK gene from Herpes simplex was, therefore, introduced into the Plasmodium falciparum 3D7 strain and the effect on BrdU labelling was assessed by enzyme-linked immunosorbent assay and immunofluorescence microscopy.ResultsIntroduction of a TK gene produces parasites that can indeed incorporate BrdU. This forms a sensitive indicator of DNA replication, which can be detected by both quantitative and qualitative assays on either a population level or a single-cell level. Plasmodium falciparum, when expressing TK, becomes unusually sensitive to BrdU toxicity.ConclusionsBrdU labelling represents a significant new tool for investigating DNA replication and the cell cycle in Plasmodium.

【 授权许可】

CC BY   
© Merrick. 2015