Hessian fly larval feeding triggers enhanced polyamine levels in susceptible but not resistant wheat

BMC Plant Biology

Research Article

Subhashree Subramanyam¹  Nagesh Sardesai²  Christie E Williams³  Subhash C Minocha⁴  Richard H Shukle⁵  Cheng Zheng⁶ 

[1] Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA;Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA;Present address: Dow AgroSciences LLC, 46268, Indianapolis, IN, USA;Department of Agronomy, Purdue University, 47907, West Lafayette, IN, USA;USDA-ARS Crop Production and Pest Control Research Unit, 47907, West Lafayette, IN, USA;Department of Biological Sciences, University of New Hampshire, 03824, Durham, NH, USA;Department of Entomology, Purdue University, 47907, West Lafayette, IN, USA;USDA-ARS Crop Production and Pest Control Research Unit, 47907, West Lafayette, IN, USA;Department of Statistics, Purdue University, 47907, West Lafayette, IN, USA;Present address: Novartis Pharmaceuticals Corporation, 07936, East Hanover, NJ, USA;

关键词: Polyamines;    Wheat;    Hessian fly;    Compatible;    Incompatible;    RT-qPCR;    Odc;    Samdc;    Spds;   

DOI  :  10.1186/s12870-014-0396-y

 received in 2014-09-18, accepted in 2014-12-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundHessian fly (Mayetiola destructor), a member of the gall midge family, is one of the most destructive pests of wheat (Triticum aestivum) worldwide. Probing of wheat plants by the larvae results in either an incompatible (avirulent larvae, resistant plant) or a compatible (virulent larvae, susceptible plant) interaction. Virulent larvae induce the formation of a nutritive tissue, resembling the inside surface of a gall, in susceptible wheat. These nutritive cells are a rich source of proteins and sugars that sustain the developing virulent Hessian fly larvae. In addition, on susceptible wheat, larvae trigger a significant increase in levels of amino acids including proline and glutamic acid, which are precursors for the biosynthesis of ornithine and arginine that in turn enter the pathway for polyamine biosynthesis.ResultsFollowing Hessian fly larval attack, transcript abundance in susceptible wheat increased for several genes involved in polyamine biosynthesis, leading to higher levels of the free polyamines, putrescine, spermidine and spermine. A concurrent increase in polyamine levels occurred in the virulent larvae despite a decrease in abundance of Mdes-odc (ornithine decarboxylase) transcript encoding a key enzyme in insect putrescine biosynthesis. In contrast, resistant wheat and avirulent Hessian fly larvae did not exhibit significant changes in transcript abundance of genes involved in polyamine biosynthesis or in free polyamine levels.ConclusionsThe major findings from this study are: (i) although polyamines contribute to defense in some plant-pathogen interactions, their production is induced in susceptible wheat during interactions with Hessian fly larvae without contributing to defense, and (ii) due to low abundance of transcripts encoding the rate-limiting ornithine decarboxylase enzyme in the larval polyamine pathway the source of polyamines found in virulent larvae is plausibly wheat-derived. The activation of the host polyamine biosynthesis pathway during compatible wheat-Hessian fly interactions is consistent with a model wherein the virulent larvae usurp the polyamine biosynthesis machinery of the susceptible plant to acquire nutrients required for their own growth and development.

【 授权许可】

Unknown   
© Subramanyam et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.