Duodenal microbiota composition and mucosal homeostasis in pediatric celiac disease

BMC Gastroenterology

Research Article

Hannu Lähteenoja¹  Marko Kalliomäki²  Jing Cheng³  Jarkko Salojärvi³  Reetta Satokari³  Airi Palva³  Willem M de Vos⁴  Hans GHJ Heilig⁵ 

[1] Department of Internal Medicine, Turku University Central Hospital, P.O. Box 52, 20521, Turku, Finland;Department of Pediatrics, University of Turku and Turku University Central Hospital, P.O. Box 52, 20521, Turku, Finland;Functional Foods Forum, 20014 University of Turku, Turku, Finland;Department of Veterinary Biosciences, University of Helsinki, P.O. Box 66, FI-00014, Helsinki, Finland;Department of Veterinary Biosciences, University of Helsinki, P.O. Box 66, FI-00014, Helsinki, Finland;Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands;Haartman Institute, University of Helsinki, P.O. Box 21, FI-00014, Helsinki, Finland;Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands;

关键词: Celiac disease;    Microbiota;    Gene expression;    Duodenum;    Host-microbe cross-talk;   

DOI  :  10.1186/1471-230X-13-113

 received in 2013-04-19, accepted in 2013-07-08,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCeliac disease (CD) is an autoimmune disorder of the small intestine which is triggered by dietary gluten in genetically predisposed (HLA-DQ2/DQ8 positive) individuals. Only a fraction of HLA-DQ2/DQ8 positive individuals develop CD indicating that other factors have a role in the disorder. Several studies have addressed intestinal microbiota aberrancies in pediatric CD, but the results are inconsistent. Previously, we demonstrated that pediatric CD patients have lower duodenal expression of TLR2 and higher expression of TLR9 as compared to healthy controls (HC) indicating that microbiota may have a role in CD.MethodsWe used bacterial phylogenetic microarray to comprehensively profile the microbiota in duodenal biopsies of CD (n = 10) and HC (n = 9) children. The expression of selected mucosa-associated genes was assessed by qRT-PCR in CD and HC children and in treated CD adults (T-CD, n = 6) on gluten free diet.ResultsThe overall composition, diversity and the estimated microbe associated molecular pattern (MAMP) content of microbiota were comparable between CD and HC, but a sub-population profile comprising eight genus-like bacterial groups was found to differ significantly between HC and CD. In HC, increased TLR2 expression was positively correlated with the expression of tight junction protein ZO-1. In CD and T-CD, the expression of IL-10, IFN-g and CXCR6 were higher as co5mpared to HC.ConclusionsThe results suggest that microbiota and altered expression of mucosal receptors have a role in CD. In CD subjects, the increased expression of IL-10 and IFN-g may have partly resulted from the increased TLR9 expression and signaling.

【 授权许可】

Unknown   
© Cheng et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.